Analytical and Bioanalytical Chemistry

, Volume 378, Issue 1, pp 84–88 | Cite as

Target-selective ion-exchange media for highly hydrophilic compounds: a possible solution by use of the “interval immobilization technique”

  • Takuya Kubo
  • Nobuo Tanaka
  • Ken HosoyaEmail author
Paper in Forefront


We prepared a crosslinked polymer as a separation and/or adsorption medium for CYN, as shown in Fig. 1. The polymers were evaluated by high-performance liquid chromatography (HPLC) and adsorption was examined under batch conditions. Results from detailed HPLC evaluation and measurement of the difference between the binding affinity for CYN and for other compounds showed the prepared polymer had specific recognition ability for CYN.


Ion-exchange media Highly hydrophilic compounds Interval immobilization technique Cylindrospermopsin (CYN) Cyanobacterium hepatotoxin 


  1. 1.
    Nilsson K, Lindell J, Norlow O, Sellergren B (1994) J Chromatogr A 680:57CrossRefGoogle Scholar
  2. 2.
    Kempe M, Mosbach K (1995) J Chromatogr A 691:317CrossRefPubMedGoogle Scholar
  3. 3.
    Kempe M, Fischer L, Mosbach K (1993) J Mol Recognit 6:25PubMedGoogle Scholar
  4. 4.
    Spivak D, Shea KJ (1999) J Org Chem 64:4627CrossRefPubMedGoogle Scholar
  5. 5.
    Haginaka J, Sanbe H (2000) Anal Chem 72:5206CrossRefPubMedGoogle Scholar
  6. 6.
    Yoshizako K, Hosoya K, Iwakoshi Y, Kimata K, Tanaka N (1998) Anal Chem 70:386CrossRefGoogle Scholar
  7. 7.
    Hosoya K, Yoshizako K, Sasaki H, Kimata K, Tanaka N (1998) J Chromatogr A 828:91CrossRefGoogle Scholar
  8. 8.
    Hosoya K, Iwakoshi Y, Yoshizako K, Kimata K, Tanaka N, Takehira H, Haginaka J (1999) J High Resolut Chromatogr 22:256CrossRefGoogle Scholar
  9. 9.
    Hosoya K, Yoshizako K, Kubo T, Ikegami T, Tanaka N, Haginaka J (2002) Anal Sci 18:55PubMedGoogle Scholar
  10. 10.
    Kubo T, Hosoya K, Watabe Y, Ikegami T, Tanaka N, Sano T, Kaya K (2003) J Chromatogr A 987:389CrossRefPubMedGoogle Scholar
  11. 11.
    Sajionz P, Kele M, Zhong G, Sellergren B, Guiochon G (1998) J Chromatogr A 810:1CrossRefGoogle Scholar
  12. 12.
    Quaglis M, Chenon K, Hall AJ, Lorenzi ED, Sellergren B (2001) J Am Chem Soc 123:2146CrossRefPubMedGoogle Scholar
  13. 13.
    Takeuchi T, Mukawa T, Matsui J, Higashi M, Shimizu KD (2001) Anal Chem 73:3869CrossRefPubMedGoogle Scholar
  14. 14.
    Kaya K, Watanabe MM (1994) Microbiol Cult Coll June, ReviewGoogle Scholar
  15. 15.
    Codd GA, Metcalf JS, Kaya K (2001) J AOAC Int 84:1626PubMedGoogle Scholar
  16. 16.
    Hunter PR (1992) J Med Microbiol 36:301PubMedGoogle Scholar
  17. 17.
    Elder GH, Hunter PR, Codd GA (1993) Lancet 341:1519CrossRefPubMedGoogle Scholar
  18. 18.
    Harada K, Ohtani I, Terao K (1994) Toxicon 32:73CrossRefPubMedGoogle Scholar
  19. 19.
    Hawkins PR, Chandrasena NR, Fralconer IR (1997) Toxicon 35:341CrossRefPubMedGoogle Scholar
  20. 20.
    Li R, Carmichael WW, Brittain S, Kaya K, Watanabe MM (2001) Toxicon 39:973CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Department of Polymer Science and EngineeringKyoto Institute of TechnologySakyo-kuJapan

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