Analytical and Bioanalytical Chemistry

, Volume 378, Issue 3, pp 664–669 | Cite as

Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors

  • Marie-Louise Scippo
  • Catherine Argiris
  • Cécile Van De Weerdt
  • Marc Muller
  • Philippe Willemsen
  • Joseph Martial
  • Guy Maghuin-Rogister
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERα, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, α- and β-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERα, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.

Keywords

Endocrine disruptors Steroid receptors Binding assay Pesticides Radioreceptor assay 
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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Marie-Louise Scippo
    • 1
  • Catherine Argiris
    • 1
  • Cécile Van De Weerdt
    • 2
  • Marc Muller
    • 2
  • Philippe Willemsen
    • 2
  • Joseph Martial
    • 2
  • Guy Maghuin-Rogister
    • 1
  1. 1.Département des Sciences des Denrées Alimentaires, Université de LiègeBâtiment B-43bisLiègeBelgium
  2. 2.Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de LiègeBâtiment de Chimie B-6LiègeBelgium

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