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Analytical and Bioanalytical Chemistry

, Volume 378, Issue 3, pp 664–669 | Cite as

Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors

  • Marie-Louise ScippoEmail author
  • Catherine Argiris
  • Cécile Van De Weerdt
  • Marc Muller
  • Philippe Willemsen
  • Joseph Martial
  • Guy Maghuin-Rogister
Original Paper

Abstract

This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERα, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, α- and β-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERα, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.

Keywords

Endocrine disruptors Steroid receptors Binding assay Pesticides Radioreceptor assay 

References

  1. 1.
    Bowerman WW, Best DA, Grubb TG, Sikarskie JG, Giesy JP (2000) Chemosphere 41:1569–1574CrossRefPubMedGoogle Scholar
  2. 2.
    Vos JG, Dybing E, Greim HA, Ladefoged O, Lambre C, Tarazona JV, Brandt I, Vethaak AD (2000) Crit Rev Toxicol 30:71–133PubMedGoogle Scholar
  3. 3.
    Guillette LJ, Gunderson MP (2001) Reproduction 122:857–864PubMedGoogle Scholar
  4. 4.
    Sharpe RM (2001) Toxicol Lett 120:221–232CrossRefPubMedGoogle Scholar
  5. 5.
    Lebel G, Dodin S, Ayotte P, Marcoux S, Ferron LA, Dewailly E (1998) Fertil Steril 69:221–228CrossRefPubMedGoogle Scholar
  6. 6.
    Ohlson C-G, Hardell L (2000) Chemosphere 40:1277–1282CrossRefPubMedGoogle Scholar
  7. 7.
    Jensen TK, Toppari J, Keiding N Skakebaek NE (1995) Clin Chem 41:1896–1901PubMedGoogle Scholar
  8. 8.
    Skakkebaek NE (1998) Adv Exp Med Biol 444:1–2PubMedGoogle Scholar
  9. 9.
    Murray TJ, Lea RG, Abramovitch DR, Haites NE, Fowler PA (2001) Early Pregnancy V:8–112Google Scholar
  10. 10.
    Jongbloet PH, Roleveld N, Groenewoud HMM (2002) Environ Health Perspect 110:1–3PubMedGoogle Scholar
  11. 11.
    Mendes JJA (2002) Food Chem Toxicol 40:781–788CrossRefPubMedGoogle Scholar
  12. 12.
    Multigner L, Oliva A (2002) Cad Saude Publica 18 :403–412Google Scholar
  13. 13.
    Soto AM, Chung LC, Sonnenschein C (1994) Environ Health Perspect 102:380–383PubMedGoogle Scholar
  14. 14.
    Soto AM, Sonnenschein C, Chung LC, Fernandez MF, Olea N, Olea Serrano F (1995) Environ Health Perspect 103:113–122Google Scholar
  15. 15.
    Whithe R Jobling S Hoare SA Sumpter JP Parker MG(1994) Endocrinology 135:175–182PubMedGoogle Scholar
  16. 16.
    Kelce WR, Stone CR, Laws SC, Gray LE, Kempainen JA, Wilson EM (1995) Nature 375:581–585PubMedGoogle Scholar
  17. 17.
    Scippo ML, Van De Weerdt C, Willemsen P, François J-M Rentier-Delrue F, Muller M, Martial JA, Maghuin-Rogister G (2002) Anal Chim Acta 473:135–141CrossRefGoogle Scholar
  18. 18.
    Kuiper GGJM, Lemmen JG, Carlsson BO, Corton C, Safe SH, van der Saag PT, van der Burg B, Gustafsson J-A (1998) Endocrinology 139:4252–4263PubMedGoogle Scholar
  19. 19.
    Ousterhout J, Struc F, Nelson JA 1981 Biochem Pharmacol 30:2869-2871Google Scholar
  20. 20.
    Klotz DM, Ladlie BL, Vonier PM, McLachlan JA, Arnold SF (1997) Mol Cell Endocrinol 129:63–71PubMedGoogle Scholar
  21. 21.
    Sumida K, Saito K, Ooe N, Isobe N, Kaneko H, Nakatsuka I (2001) Toxicol Lett 118:147–155CrossRefPubMedGoogle Scholar
  22. 22.
    Soto AM, Justicia H, Wray JW, Sonnenschein C (1991) Environ Health Perspect 92:167–173PubMedGoogle Scholar
  23. 23.
    Vonier PM, Crain DA, MacLachlan JA, Guillette LJ, Arnold SF (1996) Environ Health Perspect 104:1318–1322PubMedGoogle Scholar
  24. 24.
    Jin L, Tran Q, Ide CF, McLachlan JA, Arnold SF (1997) Biochem Biophys Res Commun 233 :139–146Google Scholar
  25. 25.
    Tran DQ, Klotz DM Ladlie BL, Ide CF, McLachlan JA, Arnold SF (1996) Biochem Biophys Res Commun 229:518–523CrossRefPubMedGoogle Scholar
  26. 26.
    Collins BM, McLachlan JA, Arnold SF (1997) Steroids 2:365–372CrossRefGoogle Scholar
  27. 27.
    Willemsen P, Scippo M-L, Maghuin-Rogister G, Martial JA, Muller M (2003) ABC, this issueGoogle Scholar
  28. 28.
    Bagga D, Anders KH, Wang H, Roberts E, Glaspy JA (2000) J Natl Cancer Inst 92:750–753CrossRefPubMedGoogle Scholar
  29. 29.
    Hoyer AP, Jorgensen T, Grandjean P, Hartvig HB (2000) Cancer Causes Control 11:177–184CrossRefPubMedGoogle Scholar
  30. 30.
    Wolff M, Zeleniuch-Jacquotte A, Dubin N, Toniolo P (2000) Cancer Epidemiol Biomarkers Prev 9:271–277PubMedGoogle Scholar
  31. 31.
    Bauer ERS, Meyer HHD, Stahlschmidt-Allner P, Sauerwein H (1998) Analyst 123:2485–2487CrossRefPubMedGoogle Scholar
  32. 32.
    Bauer ERS, Bitsch N, Brunn H, Sauerwein H, Meyer HHD (2002) Chemosphere 46:1107–1115CrossRefPubMedGoogle Scholar
  33. 33.
    Kelce WR, Wilson EM (1997) J Mol Med 75:198–207CrossRefPubMedGoogle Scholar
  34. 34.
    Sohoni P Sumpter JP (1998) J Endocrinol 158 :327–339Google Scholar
  35. 35.
    Maness SC, McDonnell DP, Gaido KW (1998) Toxicol Appl Pharmacol 151:135–142PubMedGoogle Scholar
  36. 36.
    Gaido KW, Maness SC, McDonnell DP, Dehal SS, Kupfer D, Safe S (2000) Mol Pharmacol 58:852–858PubMedGoogle Scholar
  37. 37.
    Klotz DM, Beckman BS, Hill SM, McLachlan JA, Walters MR Arnold SF (1996) Environ Health Perspect 104:1084–1089PubMedGoogle Scholar
  38. 38.
    Krishnan AV, Stathis P, Permuth SF, Tokes L, Feldman D (1993) Endocrinology 132:2279–2286PubMedGoogle Scholar
  39. 39.
    Stelzer A, Chan HM (1999) Toxicology 138:69–80CrossRefPubMedGoogle Scholar
  40. 40.
    Kelce WR, Monosson E, Gamcsik MP, Laws SC, Gray LE (1994) Toxicol Appl Pharmacol 126:276–285CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Marie-Louise Scippo
    • 1
    Email author
  • Catherine Argiris
    • 1
  • Cécile Van De Weerdt
    • 2
  • Marc Muller
    • 2
  • Philippe Willemsen
    • 2
  • Joseph Martial
    • 2
  • Guy Maghuin-Rogister
    • 1
  1. 1.Département des Sciences des Denrées Alimentaires, Université de LiègeBâtiment B-43bisLiègeBelgium
  2. 2.Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de LiègeBâtiment de Chimie B-6LiègeBelgium

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