Skip to main content
SpringerLink
Log in

Chemometrics-assisted simultaneous determination of atenolol and chlorthalidone in synthetic binary mixtures and pharmaceutical dosage forms

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Resolution of binary mixtures of atenolol (ATE) and chlorthalidone (CTD) with minimum sample pre-treatment and without analyte separation has been successfully achieved, using a new and rapid method based on partial least squares (PLS1) analysis of UV spectral data. The simultaneous determination of both analytes was possible by PLS1 processing of sample absorbances between 255 and 300 nm for ATE and evaluation of absorbances in the 253–268 nm region for CTD. The mean recoveries for synthetic samples were 100.3±1.0% and 100.7±0.7% for ATE and CTD, respectively. Application of the proposed method to two commercial tablet preparations in the content uniformity test showed them to contain 103.5±0.8% and 104.9±1.8% ATE respectively, as well as 103.4±1.2% and 104.5±2.2% CTD. Use of this method also allowed the elaboration of dissolution profiles of the drugs in two commercial combined formulation products, through the simultaneous determination of both drugs during the dissolution test. At the dissolution time of 45 min specified by USP XXIV, both pharmaceutical formulations complied with the test.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

References

  1. (1993) Fifth report of the Joint National Committee on Detection, Evaluation and Treatment of High Blood Pressure (JNC V). Arch Intern Med 153:154

    Article  PubMed  Google Scholar 

  2. Materson BJ, Reda DJ, Cushman DC, Henderson WG (1995) J Hum Hypertens 9:791

    CAS  PubMed  Google Scholar 

  3. Dept. Health (2000) British Pharmacopoeia. Her Majesty's Stationary Office, London, UK, p49, 134

  4. (1997) European Pharmacopoeia 3rd edn. Council of Europe, Strasbourg Cedex, France, pp418–419, 614–615

  5. (2000) United States Pharmacopoeia XXIV edn. The USP Convention, Rockville, MD, USA, pp402–403, 175–176, 176–177, 2151

  6. Sa'sa SI, Jalal IM, Khalil HS (1988) J Liq Chromatogr 11:1673

    CAS  Google Scholar 

  7. Ficarra R, Ficarra P, Tommasini A, Calabro ML, Guarniera-Fenech C (1985) Farmaco 40:307

    CAS  Google Scholar 

  8. Bonazzi D, Gotti R, Andrisano V, Cavrini V (1996) Farmaco 51:733

    CAS  Google Scholar 

  9. Giachetti C, Tenconi A, Canali S, Zanolo G (1997) J Chromatogr B 698:187

    Article  CAS  Google Scholar 

  10. Dadgar D, Kelly MT (1988) Analyst 113:1223

    CAS  PubMed  Google Scholar 

  11. Gong L (1989) Yaowu Fenxi Zazhi 9:175

    CAS  Google Scholar 

  12. Wehner W (2000) Pharmazie 55:543

    CAS  PubMed  Google Scholar 

  13. Xu L, Schechter I (1996) Anal Chem 68:2392

    Article  CAS  Google Scholar 

  14. Ferraro MCF, Castellano PM, Kaufman TS (2001) J Pharm Biomed Anal 26:443

    Article  CAS  PubMed  Google Scholar 

  15. Martens H, Naes T (1989) Multivariate calibration. Wiley, Chichester, UK

  16. Thomas EV (1994) Anal Chem A 66:795

    Google Scholar 

  17. Navalon A, Blanc R, del Olmo M, Vilchez JL (1999) Talanta 48:469

    Article  CAS  Google Scholar 

  18. Guiberteau A, Galeano T, Espinosa-Mansilla A, de Alba P L, Salinas F (1995) Anal Chim Acta 302:9

    Article  Google Scholar 

  19. Rupprecht M, Probst T (1997) Fresenius J Anal Chem 359:442

    Article  CAS  Google Scholar 

  20. Wold H, Martens H, Wold S (1983) In: Ruhe A, Kagstrom B (eds) The Multivariative calibration problem in chemistry solved by the PLS method. Springer, Heidelberg, p286

  21. Haaland DM, Thomas EV (1988) Anal Chem 60:1193

    CAS  Google Scholar 

  22. Banakar UV, Lathia CD, Wood JH (1992) In: Banakar UV (ed) Pharmaceutical dissolution testing. Marcel Dekker, New York, pp189–250

  23. Chen CS, Brown CW (1994) Pharm Res 11:979

    Article  CAS  PubMed  Google Scholar 

  24. Murtha JL, Julian TN, Radebaugh GW (1988) J Pharm Sci 77:715

    CAS  PubMed  Google Scholar 

  25. Surmeian M (1998) Drug Dev Ind Pharm 24:691

    CAS  PubMed  Google Scholar 

  26. Banoglu E, Ozkan Y, Atay O (2000) Farmaco 55:477

    Article  CAS  PubMed  Google Scholar 

  27. Mannucci C, Bertini J, Cocchini A, Perico A, Salvagnini F, Triolo A (1992) J Pharm Sci 81:1175

    CAS  PubMed  Google Scholar 

  28. Ferraro MCF, Castellano PM, Kaufman TS (2002) J Pharm Biomed Anal 30:1121

    Article  CAS  PubMed  Google Scholar 

  29. Dinç E, Serin C, Tugcu-Demiröz F, Doganay T (2003) Int J Pharm 250:339

    Article  PubMed  Google Scholar 

  30. Langenbucher F (1972) J Pharm Pharmacol 24:979

    CAS  PubMed  Google Scholar 

  31. Nelson KG, Wang LY (1970) J Pharm Sci 66:1758

    Google Scholar 

  32. Nelson KG, Wang LY (1970) J Pharm Sci 66:86

    Google Scholar 

Download references

Acknowledgements

The authors thank UNR for financial support, Dr. Alejandro C. Olivieri for providing access to the Jasco V-530 UV-Vis spectrophotometer, Droguería Prest for the donation of atenolol, and Dr. Lucio Jeroncic for the kind provision of chlorthalidone. T.S.K. is also grateful to CONICET.

Author information

Authors and Affiliations

  1. Area Análisis de Medicamentos, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina

    Mónica C. F. Ferraro, Patricia M. Castellano & Teodoro S. Kaufman

  2. Instituto de Química Orgánica de Síntesis -IQUIOS-(CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina

    Teodoro S. Kaufman

Authors
  1. Mónica C. F. Ferraro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patricia M. Castellano
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Teodoro S. Kaufman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Teodoro S. Kaufman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferraro, M.C.F., Castellano, P.M. & Kaufman, T.S. Chemometrics-assisted simultaneous determination of atenolol and chlorthalidone in synthetic binary mixtures and pharmaceutical dosage forms. Anal Bioanal Chem 377, 1159–1164 (2003). https://doi.org/10.1007/s00216-003-2185-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-003-2185-6

Keywords

Search

Navigation