Advertisement

Analytical and Bioanalytical Chemistry

, Volume 377, Issue 1, pp 208–213 | Cite as

Separation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high-performance liquid chromatography

  • Jia-Ping Lai
  • Xi-Wen He
  • Feng ChenEmail author
Original Paper

Abstract

Molecularly imprinted microspheres were synthesised using the antitumor drug piritrexim (PTX) as a template molecule by aqueous microsuspension polymerisation and were used as a high-performance liquid chromatographic stationary phase. The molecularly imprinted column exhibited strong retention behaviour to the template molecule. The influences of pH of the buffer and the ratio of methanol to buffer on the retention behaviour were investigated in detail. Results indicated that the baseline separation of PTX, trimetrexate (TMX), trimethoprim (TMP) and sulfamethazine (SMZ) was achieved when the pH value of the acetate buffer was above pH 3.5 and the ratio of methanol to the buffer was 6:4 (v/v). A gradient elution programme was employed to enhance the separation, which led to an improvement in sensitivity and a reduction in determination errors. The method developed was used to analyse urine samples supplemented with PTX. The recoveries of 5 μg mL−1 PTX in the urine sample were 99.1±3.0% and 93.3±2.8% at the beginning and 24 h later, respectively.

Keywords

Molecularly imprinted microspheres Aqueous microsuspension polymerisation Chromatographic stationary phase Piritrexim Trimetrexate 

Notes

Acknowledgements

This research was supported by the CRCG (The University of Hong Kong Committee on Research and Conference Grants), RGC (the Hong Kong Research Grants Council) and the National Natural Science Foundation of China (No. 29775014, No. 20175009).

References

  1. 1.
    Dewit R, Kaye SB, Roberts JT, Stoter G, Scott J, Verweij J (1993) British J Cancer 67:388–390Google Scholar
  2. 2.
    Zhang WN, Li WH, Zhen QY (1999) In: Rui YC, Rui YC (eds) Xiandai Yaowuxue. Renming Junyi, Beijing, p 1121, chap 59Google Scholar
  3. 3.
    Ackerly CC, Hartshorn J, Tong WP, Mccormack JJ (1985) J Liq Chromatogr 8:125–134Google Scholar
  4. 4.
    Tinsley PW, Lacreta FP (1990) J Chromatogr Biomed Appl 529:468–472CrossRefGoogle Scholar
  5. 5.
    Stetson PL, Ensminger WD (1986) J Chromatogr 383:69–76CrossRefPubMedGoogle Scholar
  6. 6.
    Mosbach K, Ramstrom O (1996) BioTechnol 14:163–170Google Scholar
  7. 7.
    Takeuchi T, Matsui J (1996) Acta Polymerica 47:471–480CrossRefGoogle Scholar
  8. 8.
    Masque N, Marce RM, Borrull F (2001) Trends Anal Chem 20:477–486CrossRefGoogle Scholar
  9. 9.
    Mullett WM, Lai EPC (1998) Anal Chem 70:3636–3641Google Scholar
  10. 10.
    Deore B, Chen ZD, Nagaoka T (1999) Anal Sci 15:827–828Google Scholar
  11. 11.
    Quaglia M, Chenon K, Hall AJ, Lorenzi ED, Sellergren B (2001) J Am Chem Soc 123:2146–2154CrossRefPubMedGoogle Scholar
  12. 12.
    Wulff G, Gross T, Schonfeld R (1997) Angew Chem Int Ed Eng 36 :1962–1964Google Scholar
  13. 13.
    Piletsky SA, Piletska EV, Chen BN, Karim K, Weston D, Barrett G, Lowe P, Turner APF (2000) Anal Chem 72:4381–4385CrossRefPubMedGoogle Scholar
  14. 14.
    Piletsky SA, Piletska EV, Bossi A, Karim K, Lowe P, Turner APF (2001) Biosen Bioelec 16:701–707CrossRefGoogle Scholar
  15. 15.
    Svensson J, Nicholls IA (2001) Anal Chim Acta 435:19–24CrossRefGoogle Scholar
  16. 16.
    Kriz D, Ramstrom O, Svensson A, Mosbach K (1995) Anal Chem 67:2142–2144Google Scholar
  17. 17.
    Piletsky SA, Piletskaya EV, Panasyuk TL, El'skaya AV, Levi R, Karube I, Wulff G (1998) Macromolecules 31:2137–2140CrossRefGoogle Scholar
  18. 18.
    Kroger S, Turner APF, Mosbach K, Haupt K (1999) Anal Chem 71:3698–3702CrossRefPubMedGoogle Scholar
  19. 19.
    Rich JO, Dordick JS (1997) J Am Chem Soc 119:3245–3252CrossRefGoogle Scholar
  20. 20.
    Ye L, Ramstrom O, Mansson MO, Mosbach K (1998) J Mol Recognit 11:75–78Google Scholar
  21. 21.
    Rashid BA, Briggs RJ, Hay JN, Stevenson D (1997) Anal Commun 34:303–305CrossRefGoogle Scholar
  22. 22.
    Bjarnason B, Chimuka L, Ramstrom O (1999) Anal Chem 71:2152–2156CrossRefGoogle Scholar
  23. 23.
    Mullett WM, Dirie MF, Lai EPC, Guo HS, He XW (2000) Anal Chim Acta 414:123–131CrossRefGoogle Scholar
  24. 24.
    Mayes AG, Mosbach K (1996) Anal Chem 68:3769–3774CrossRefGoogle Scholar
  25. 25.
    Hosoya K, Yoshizako K, Shirasu Y, Kimata K, Araki T, Tanaka N, Haginaka J (1996) J Chromatogr A 728:139–147CrossRefGoogle Scholar
  26. 26.
    Schweitz L, Andersson LI, Nilsson S (1997) Anal Chem 69:1179–1183CrossRefGoogle Scholar
  27. 27.
    Hosoya K, Shirasu Y, Kimata K, Tanaka N (1998) Anal Chem 70:943–945CrossRefGoogle Scholar
  28. 28.
    Haginaka J, Takehira H, Hosoya K, Tanaka N (1999) J Chromatogr A 849:331–339CrossRefPubMedGoogle Scholar
  29. 29.
    Yu C, Mosbach K (2000) J Chromatogr A 888:63–72CrossRefPubMedGoogle Scholar
  30. 30.
    Lai JP, Lu XY, Lu CY, Ju HF, He XW (2001) Anal Chim Acta 442 105–111Google Scholar
  31. 31.
    Lele BS, Kulkarni MG, Mashekar RA (1999) React Funct Polm 39:37–52CrossRefGoogle Scholar
  32. 32.
    Zhou J, He XW, Guo HS (2000) Chinese J Chem 18:482–488Google Scholar
  33. 33.
    Markowitz MA, Kust PR, Klaehn J, Deng G, Gaber BP (2001) Anal Chim Acta 435:177–185CrossRefGoogle Scholar
  34. 34.
    Graffner-Nordberg M, Kolmodin K, Aqvist J, Queener SF, Hallberg A (2001) J Med Chem 44:2391–2402CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Department of BotanyThe University of Hong KongHong KongChina
  2. 2.Department of ChemistryNankai UniversityTianjinChina

Personalised recommendations