Analytical and Bioanalytical Chemistry

, Volume 377, Issue 1, pp 189–194 | Cite as

Determination of ATP via the photochemical generation of hydrogen peroxide using flow injection luminol chemiluminescence detection

  • Tomás Pérez-Ruiz
  • Carmen Martínez-Lozano
  • Virginia Tomás
  • Jesús Martín
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

The determination of ATP using the coupling between a photochemical reaction and a chemiluminescence reaction in a flow injection (FI) system is described. The method is based on the reaction of glucose with ATP catalyzed by hexokinase and Mg2+ ions. The glucose that is not consumed by ATP is subsequently photooxidized using 9,10-anthraquinone-2,6-disulfonate as a sensitizer. The hydrogen peroxide produced in the photochemical reaction is monitored through the chemiluminescence reaction with luminol catalyzed by hematine. There is a linear relationship between the decrease in the chemiluminescence response and the ATP concentration at a constant concentration of glucose. Under the optimum conditions, the calibration graph is linear in the range 0.20–50.5 mg L−1 with a throughput of 25 samples per hour and relative standard deviations between ±0.62 and ±1.42%. The limit of detection is 0.07 mg L−1. The method was used for the determination of ATP in pharmaceuticals, milk, and soils.

Keywords

ATP 9,10-Anthraquinone-2,6-disulfonate Photochemical reaction Flow injection Chemiluminescence detection 
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Notes

Acknowledgements

Financial support from Fundación Séneca (Comunidad Autónoma de Murcia, PI-62/0556/FS/01) and Ministerio de Ciencia y Tecnología is acknowledged. Jesús Martín holds a fellowship from CajaMurcia (Spain).

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Tomás Pérez-Ruiz
    • 1
  • Carmen Martínez-Lozano
    • 1
  • Virginia Tomás
    • 1
  • Jesús Martín
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of ChemistryUniversity of MurciaSpain

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