Analytical and Bioanalytical Chemistry

, Volume 376, Issue 7, pp 1041–1052 | Cite as

Determination of protonation constants of some fluorinated polyamines by means of 13C NMR data processed by the new computer program HypNMR2000. Protonation sequence in polyamines

  • Chiara Frassineti
  • Lucia Alderighi
  • Peter Gans
  • Antonio Sabatini
  • Alberto Vacca
  • Stefano Ghelli
Special Issue Paper


The pKa values of 6-fluoro-4,8-diazadodecane-1,12-diamine (6-fluorospermine) (1), 6,6-difluoro-4,8-diazadodecane-1,12-diamine (6,6-difluorospermine) (2), 6-fluoro-4-azaoctane-1,8-diamine (6-fluorospermidine) (3) and 6,6-difluoro-4-azaoctane-1,8-diamine (6,6-difluorospermidine) (4) in D2O solution have been determined at 40 °C from 13C NMR chemical shifts data using the new computer program HypNMR2000. The enthalpies of protonation of compounds 1–4 and the parent amines spermine (5) and spermidine (6) have been determined from microcalorimetric titration data. The values of ΔH° were used to derive basicity constants relative to 25 °C. The NMR data have been analysed by two different methods to obtain information on the protonation sequence in the polyamines 1–5. The protonation sequence for spermine is related to its biological activity.


13C NMR Fluorinated compounds Polyamines Protonation constants Protonation enthalpies Protonation sequence 



Protein kinase C




Microsoft Visual Basic



The Italian Ministry for Education, University and Research, MIUR, and the Italian National Council for Research, CNR, are gratefully acknowledged for funding. The authors are grateful to Centro Interdipartimentale Grandi Strumenti dell'Università di Modena, for allowing the use of the AMX-400 WB Bruker spectrometer. We thank Dr Francesca Benevelli, formerly at the Centro Grandi Strumenti dell'Università di Pavia, for technical assistance with the NMR measurements.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Chiara Frassineti
    • 1
  • Lucia Alderighi
    • 3
  • Peter Gans
    • 2
  • Antonio Sabatini
    • 3
  • Alberto Vacca
    • 3
  • Stefano Ghelli
    • 4
  1. 1.Dipartimento di Scienze BiomedicheUniversità degli Studi di ModenaModenaItaly
  2. 2.Department of ChemistryThe University of LeedsLeedsEngland
  3. 3.Dipartimento di ChimicaUniversità degli Studi di Firenze Sesto FiorentinoItaly
  4. 4.SPIN Rubiera (Reggio Emilia)Italy

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