Analytical and Bioanalytical Chemistry

, Volume 377, Issue 1, pp 32–38 | Cite as

Preparation, homogeneity and stability studies of a candidate LRM for Se speciation

  • Erzsébet Tünde Bodó
  • Zsolt Stefánka
  • Ildikó Ipolyi
  • Csilla Sörös
  • Mihály Dernovics
  • Péter Fodor
Special Issue Paper

Abstract

A laboratory reference material (LRM) was prepared from Brazil nuts (Bertholletia excelsa) for quality control (QC) purposes of selenium speciation. The preparation of this LRM led through the usual operation steps applied during routine reference material production from biota samples—preparation of the raw material, homogenisation, storage design, checking of homogeneity, microbiological status and possible irradiation effects, and monitoring the species stability vs time at different storage temperatures. The selenium speciation studies to check species stability were carried out on a HPLC–UV–HG–AFS measurement set-up. Special attention was paid to the correct identification of selenium species by applying independent HPLC separation techniques (ion-pairing and anion-exchange chromatography). The concentration of selenomethionine (SeMet) and total Se content were quantified (79.9 µg g−1 (calculated as Se) and 82.9 µg g−1, respectively). The homogeneity and stability of this candidate reference material passed the relevant tests recommended by Bureau Communautaire de Référence (BCR).

Keywords

Selenium speciation Laboratory reference material Selenomethionine 

Notes

Acknowledgements

The financial support of OTKA (Hungarian Scientific Foundation; T37216 and F32296) is gratefully acknowledged. E.T. Bodó acknowledges a fellowship from Bay Zoltán Foundation (Budapest, Hungary). The authors express their gratitude to Zsuzsanna Csabai and Csilla Szabó for their contribution to sample preparation, to Professor Hlavay for the loan of the AFS detector and to the Department of Microbiology of the Central Food Research Institute (Budapest, Hungary) for microbiological determinations and γ-irradiation.

References

  1. 1.
    Quevauviller Ph, Cámara C, Palacios MA, Cobo MG, Munoz R (1997) Se(IV) and Se(VI) species and total Se in two artificial freshwater solutions, EUR Report, 18044 EN. European Commission, Brussels, BelgiumGoogle Scholar
  2. 2.
    MULSPOT certification exercise on species of As, Hg and Sn, and on Se in oyster tissue, sample T34 (candidate BCR-710), EC Contract SM&T CT98-2232Google Scholar
  3. 3.
    McSheehy S, Yang W, Pannier F, Szpunar J, Łobiński R, Auger J, Potin-Gautier M (2000) Anal Chim Acta 42:147–153CrossRefGoogle Scholar
  4. 4.
    Whanger PD, Ip C, Polan CE, Uden PC, Welbaum G (2000) J Agric Food Chem 48:5723–5730PubMedGoogle Scholar
  5. 5.
    Montes-Bayón M, Yanes EG, Ponce de León C, Jayasimhulu K, Stalcup A, Shann J, Caruso JA (2002) Anal Chem 74:107–113PubMedGoogle Scholar
  6. 6.
    Chassaigne H, Vacchina V, Łobiński R (2000) Trends Anal Chem 19:300–313Google Scholar
  7. 7.
    Casiot C, Szpunar J, Łobiński R, Potin-Gautier M (1999) J Anal At Spectrom 14:645–650Google Scholar
  8. 8.
    Kotrebai M, Birringer M, Tyson JF, Block E, Uden PC (1999) Anal Commun 36:249–252Google Scholar
  9. 9.
    Dernovics M, Stefánka Zs, Fodor P (2002) Anal Bioanal Chem 372:473–480CrossRefPubMedGoogle Scholar
  10. 10.
    Chang JC, Gutenmann WH, Reid CM, Lisk DJ (1995) Chemosphere 30:801–802CrossRefPubMedGoogle Scholar
  11. 11.
    Secor CL, Lisk DJ (1990) J Food Safety 9:279–281Google Scholar
  12. 12.
    Kannamkumarath SS, Wrobel K, Wrobel K, Vonderheide A, Caruso JA (2002) Anal Bioanal Chem 373:454–460PubMedGoogle Scholar
  13. 13.
    Tu HM, Godfrey LW, Sun SSM (1998) Plant Mol Biol 37:829–838PubMedGoogle Scholar
  14. 14.
    Quevauviller Ph (1998) Spectrochim Acta B 53:1261–1279CrossRefGoogle Scholar
  15. 15.
    Korhammer S, Herzig R, Schramel P, Kumpulainen J, Markert B, Muntau H, Quevauviller Ph (2000) Accred Qual Assur 5:238–242CrossRefGoogle Scholar
  16. 16.
    Lamberty A, Schimmel H, Pauwels J (1998) Fresenius J Anal Chem 360:359–361Google Scholar
  17. 17.
    Vilanó M, Rubio R (2000) J Anal At Spectrom 15:177–180Google Scholar
  18. 18.
    Ipolyi I, Corns W, Stockwell P, Fodor P (2001) J Autom Method Manag 23:167–172CrossRefGoogle Scholar
  19. 19.
    Candlish AAG, Pearson SM, Aidoo KE, Smith JE, Kelly B, Irvine H (2001) Food Addit Contam 18:129–136CrossRefPubMedGoogle Scholar
  20. 20.
    Gilon N, Astruc A, Astruc M, Potin-Gautier M (1995) Appl Organomet Chem 9:623–628Google Scholar
  21. 21.
    Kramer KJM, Kramer GN, Muntau H (2003) Practical manual for the production of laboratory reference materials, 2nd edn. Mermayde, Bergen, The Netherlands, pp 36–38Google Scholar
  22. 22.
    BIPM, IEC, IFCC, ISO, IUPAC, IUPAP, OIML (1995) Guide to the expression of uncertainty in measurement, 1st edn. ISO Geneva, SwitzerlandGoogle Scholar
  23. 23.
    Pauwels J, van der Veen AMH, Lamberty A, Schimmel H (2000) Accred Qual Assur 5:95–99Google Scholar
  24. 24.
    van der Veen AMH, Linsinger T, Pauwels J (2001) Accred Qual Assur 6:26–30CrossRefGoogle Scholar
  25. 25.
    Pauwels J, Lamberty A, Schimmel H (1998) Accred Qual Assur 3:51–55CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Erzsébet Tünde Bodó
    • 1
  • Zsolt Stefánka
    • 1
  • Ildikó Ipolyi
    • 1
  • Csilla Sörös
    • 1
  • Mihály Dernovics
    • 1
  • Péter Fodor
    • 1
  1. 1.Department of Applied Chemistry, Faculty of Food ScienceSzent István UniversityBudapestHungary

Personalised recommendations