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Analytical and Bioanalytical Chemistry

, Volume 375, Issue 8, pp 1006–1010 | Cite as

Comparison of potassium ion preference of potassium-sensing oligonucleotides, PSO-1 and PSO-2, carrying the human and Oxytricha telomeric sequence, respectively

  • Shigeori TakenakaEmail author
  • Hiroyuki Ueyama
  • Takahiko Nojima
  • Makoto Takagi
Special Issue Paper

Abstract

Human [G3(TTAG3)3] and Oxytricha [G4(T4G4)3] telomere model oligonucleotides, PSO-1 and PSO-2, bearing two fluorophores, 6-carboxyfluorescein (6-FAM) and 6-carboxytetramethylrhodamine (6-TAMRA) at their 5′- and 3′-termini, respectively, were synthesized. Both of them can form an intramolecular antiparallel tetraplex upon addition of K+, and an enhanced fluorescence resonance energy transfer (FRET) was observed. PSO-1 showed a 43,000 times higher selectivity for K+ against Na+. Fluorometric and circular dichroism spectrophotometric studies revealed that this system is useful for the evaluation of the interaction of different telomeric repeat oligonucleotide sequences with metal ions.

Keywords

Fluorescence resonance energy transfer (FRET) G-quartet Potassium-sensing oligonucleotide (PSO) Telomere Tetraplex 

Notes

Acknowledgment

The authors are grateful to Prof Hiroki Kondo of the Kyushu Institute of Technology for helpful discussions. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The authors are also grateful for the financial support from the Japan Society for the Promotion of Science.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Shigeori Takenaka
    • 1
    Email author
  • Hiroyuki Ueyama
    • 1
  • Takahiko Nojima
    • 1
  • Makoto Takagi
    • 1
  1. 1.Department of Applied Chemistry, Faculty of EngineeringKyushu UniversityFukuoka 812–8581Japan

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