Analytical and Bioanalytical Chemistry

, Volume 375, Issue 8, pp 1038–1044 | Cite as

The use of thermal lensing for the determination of pyrogens

  • N. V. Orlova
  • M. A. Proskurnin
  • V. A. Samburova
  • I. D. Dryagleva
  • A. V. Brusnichkin
Special Issue Paper
First Online:
Received:
Revised:
Accepted:

Abstract.

Based on the optimized spectrophotometric determination of pyrogens (of various classes (p-aminophenol and endotoxins), thermal lensing was applied to the determination of these substances at the submicrogram level. The limit of detection of p-aminophenol, a pyrogenic impurity in pharmaceutical formulations of paracetamol, by reaction with resorcinol in alkaline solutions is 100 ng mL–1. Phloroglucinol was considered as an analog of resorcinol as a reagent in this reaction. The conditions of spectrophotometric determination of pyrogenic lipopolysaccharides (endotoxins) by ion-pair formation with methylene blue (the limit of detection is 100 ng mL–1), by ion-pair formation with Stains-All (1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide) (the limit of detection is 500 ng mL–1), and by reaction of 2-keto-3-deoxyoctonic acid with thiobarbituric acid (the limit of detection is 800 ng mL–1) were proposed. The optimized procedure for 2-keto-3-deoxyoctonic acid was applied for thermal lensing that provided a decrease in the limit of detection to 70 ng mL–1 and was also used for lipopolysaccharide determination in the endotoxin standard from E. coli.

Keywords

Thermal lensing Pyrogens Endotoxins Lipopolysaccharides p-Aminophenol 

Abbreviations

KDO

2-Keto-3-deoxyoctonic acid

LAL

Limulus amebocyte lysate

LPS

Lipopolysaccharide

TLS

Thermal lens spectrometry

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References

  1. 1.
    Yarovaya LM, Aleshin BA (1991) J Microbiol 2:74Google Scholar
  2. 2.
    Ispytanie na pirogennost (1999) Farmateka 3:21Google Scholar
  3. 3.
    Singer CJ, Underwood EA (1962) A short history of medicine. Oxford University Press, Oxford, and references cited thereinGoogle Scholar
  4. 4.
    Bialkowski SE (1996) Photothermal spectroscopy methods for chemical analysis. Wiley, New York, and references cited thereinGoogle Scholar
  5. 5.
    Snook RD, Lowe RD (1995) Analyst 120:2051Google Scholar
  6. 6.
    Georges J (1999) Talanta 48:501CrossRefGoogle Scholar
  7. 7.
    Proskurnin MA, Abroskin AG, Radushkevich DYu (1999) J Anal Chem (Moscow) 54:91Google Scholar
  8. 8.
    Khimiya Nekotorykh Grupp Sinteticheskikh Lekarstvennykh Sredstv, Part 2 (1988) Nauka, MoscowGoogle Scholar
  9. 9.
    British pharmacopoeia (1988) 414, 547, 743, 1047Google Scholar
  10. 10.
    Japan pharmacopoeia I (1982) 29Google Scholar
  11. 11.
    Gosudarstvennaya Farmakopeya RF (2001) XII editionGoogle Scholar
  12. 12.
    De La Guardia M, Khalaf KD, Hasan BA, Morales-Rubio A (1996) Analyst 121:1321Google Scholar
  13. 13.
    Proskurnin MA, Orlova NV, Pikhtar' AV, Kuznetsova VV (2001) Vestn Mosk Univ Ser 2 Khim 42:30Google Scholar
  14. 14.
    Proskurnin MA, Orlova NV, Fedyaev NV, Samburova VA, Kuznetsova VV (2001) Anal Sci 17:i1299Google Scholar
  15. 15.
    Sidorenko SV (2000) Infektsii i Antimikrobnaya Terapiya 2:23Google Scholar
  16. 16.
    Sitnikova AG, Travina LA, Bagirova VL (1997) LAL-test: Sovremennye podkhody k opredeleniyu pirogennosti TSNII, MoscowGoogle Scholar
  17. 17.
    The horseshoe crab natural history, anatomy, conservation and current research; http://www.horseshoecrab.org/Google Scholar
  18. 18.
    Soedjak HS (1994) J Anal Chem 66:4514Google Scholar
  19. 19.
    Janda J, Work E (1971) FEBS Lett 16:343CrossRefPubMedGoogle Scholar
  20. 20.
    Mikheeva MN, Gorshkova RP, Brutko LI (1988) Khim Pripod Soed 1:29Google Scholar
  21. 21.
    Chernov AB, Markov EY (1998) Russian Federation patent no 2114436Google Scholar
  22. 22.
    Westphal O, Luederitz O (1954) Angew Chem 66:407Google Scholar
  23. 23.
    Egerev SV, Zozulya OM, Pashin AE, Puchenkov OV (1992) In: Bicanic D (ed) Photoacoustic and photothermal phenomena III, Springer series in optical sciences, vol 69, Springer, Berlin Heidelberg New York, 50Google Scholar
  24. 24.
    Petsch D, Anspach FB (2000) J Biotechnol 76:98CrossRefGoogle Scholar
  25. 25.
    Frecer V, Ho B (2000) Biochem Biophys Acta 146:87Google Scholar
  26. 26.
    Waravdekar V, Saslaw D (1959) J Biol Chem 234:1945Google Scholar
  27. 27.
    Galanos C, Luberitz O (1977) Int Rev Biochem 14:239Google Scholar
  28. 28.
    Weissbach A, Hurwitz J (1959) J Biol Chem 234:705Google Scholar
  29. 29.
    Droge W, Lehmann V, et al (1970) Eur J Biochem 14:175Google Scholar
  30. 30.
    Chernysh VV, Kononets MY, Proskurnin MA, Pakhomova SV, Komissarov VV, Zatsman AI (2001) Fresenius J Anal Chem 369:535CrossRefPubMedGoogle Scholar
  31. 31.
    Associates of Cape Cod Inc. http://www.acciusa.com/index.htmlGoogle Scholar
  32. 32.
    Associates of Cape Cod Inc (2000) Product catalogGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • N. V. Orlova
    • 1
  • M. A. Proskurnin
    • 1
  • V. A. Samburova
    • 1
  • I. D. Dryagleva
    • 1
  • A. V. Brusnichkin
    • 1
  1. 1.Chemistry DepartmentM.V. Lomonosov Moscow State University MoscowRussia

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