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Analytical and Bioanalytical Chemistry

, Volume 375, Issue 1, pp 120–124 | Cite as

Multi-analyte analysis system using an antibody-based biochip

  • Maria Moreno-Bondi
  • Jean Alarie
  • Tuan Vo-Dinh
Original Paper

Abstract.

A multi-analyte detection system using a unique antibody (Ab) biochip is described. The Ab-based biochip, also referred to as the protein biochip, uses a sensor array based on a complementary metal oxide silicon (CMOS) integrated circuit. The Ab-biochip has a sampling platform of four-by-four microarrays of antibodies deposited onto a Nylon membrane substrate. The micro-arrayed antibodies can be interrogated simultaneously or sequentially using the biochip sensing array detector with the use of a diffractive optical element illuminating each antibody spot individually. The usefulness of the Ab biochip is illustrated by the measurements of immunoglogulin G (IgG) used as the model analyte system. The detection limit for Cy5-labeled IgG molecules was 13 pg.

Immunoassay Antibody array Biochip Protein biochip Multi-analyte 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Maria Moreno-Bondi
    • 1
  • Jean Alarie
    • 1
  • Tuan Vo-Dinh
    • 1
  1. 1.Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831-6101, USA

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