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Analytical and Bioanalytical Chemistry

, Volume 374, Issue 1, pp 39–45 | Cite as

Detection of organophosphorus insecticides with immobilized acetylcholinesterase – comparative study of two enzyme sensors

  • Silvana Andreescu
  • Alina Avramescu
  • Camelia Bala
  • Vasile Magearu
  • Jean-Louis Marty
Special Issue Paper

Abstract.

Two-enzyme systems based on acetylcholinesterase (AChE) – a mono-enzyme system based on AChE, with p-aminophenyl acetate as substrate, and a bi-enzyme system based on AChE and tyrosinase, with phenyl acetate as substrate – have been studied for detection of organophosphate insecticides. The analytical performance and detection limits for determination of the pesticides were compared for the two AChE configurations. The enzyme loading, pH, and applied potential of the bi-enzyme system were optimised. When phenyl acetate was used as substrate for AChE activity the phenol generated by enzymatic hydrolysis was determined with a second enzyme, tyrosinase. Amperometric measurements were performed at 100 mV and –150 mV relative to the Ag/AgCl reference electrode for the mono-enzyme and bi-enzyme systems. Screen-printed sensors were used to detect the organophosphorus pesticides paraoxon and chlorpyrifos ethyl oxon; the detection limits achieved with phenyl acetate as substrate were 5.2×10–3 mg L–1 and 0.56×10–3 mg L–1, respectively.

Substrates of acetylcholinesterase Tyrosinase Insecticides Inhibition Screen-printed biosensor 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Silvana Andreescu
    • 1
  • Alina Avramescu
    • 1
  • Camelia Bala
    • 2
  • Vasile Magearu
    • 2
  • Jean-Louis Marty
    • 1
  1. 1.Centre de Phytopharmacie, Université de Perpignan, UMR CNRS 5054, 52, Avenue de Villeneuve, 66860 Perpignan Cedex, France
  2. 2.Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4–12 Regina Elisabeta Blvd., 703461 Bucharest-1, Romania

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