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Analytical and Bioanalytical Chemistry

, Volume 373, Issue 8, pp 691–695 | Cite as

Flow-injection amperometric determination of pesticides on the basis of their inhibition of immobilized acetylcholinesterases of different origin

  • G. Jeanty
  • A. Wojciechowska
  • J.-L. Marty
  • M. Trojanowicz
Special Issue Paper

Abstract.

Determination of the organophosphorus pesticides paraoxon, chlorpyrifos oxon, and malaoxon has been performed by a method based on inhibition of acetylcholinesterase (AChE) and amperometric detection in a flow-injection system with enzymes obtained from the electric eel (eeAChE) and Drosophila melanogaster (dmAChE) and immobilized on the surface of platinum electrode within a layer of poly(vinyl alcohol) bearing styrylpyridinium groups. dmAChE is more sensitive than eeAChE to inhibition by chlorpyrifos oxon and paraoxon. The sensitivity difference was largest for chlorpyrifos oxon (detection limit approx. 17 times lower), and practically none for malaoxon. Determination of the analytes in spiked river water samples by use of the dmAChE biosensor resulted in recoveries from 50 to 90 % for chlorpyrifos oxon at levels of 20 to 40 nmol L–1, 50 to 100 % for paraoxon at 0.6 to 0.8 µmol L–1, and 140 to 190 % for malaoxon at 0.6 to 1.2 µmol L–1.

Biosensors Flow-injection analysis Amperometric detection Pesticides 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • G. Jeanty
    • 1
  • A. Wojciechowska
    • 2
  • J.-L. Marty
    • 1
  • M. Trojanowicz
    • 2
  1. 1.Centre for Phytopharmacy, University of Perpignan, CNRS 5054, 52 Avenue de Villeneuve, 66860Perpignan CedexFrance
  2. 2.Laboratory for Flow Analysis and Chromatography, Department of Chemistry, Warsaw University, Pasteura 1, 02093WarsawPoland

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