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Analytical and Bioanalytical Chemistry

, Volume 372, Issue 2, pp 391–396 | Cite as

Chromatographic characterization of molecularly imprinted microspheres for the separation and determination of trimethoprim in aqueous buffers

  • Jia-Ping Lai
  • Xian-Feng Cao
  • Xiu-Ling Wang
  • Xi-Wen He
Original Paper

Abstract.

Molecularly imprinted microspheres (MIMs) against trimethoprim (TMP), prepared by aqueous micro-suspension polymerization, bound strongly to TMP, by electrostatic and other non-covalent interactions. The effects of pH, kind and ionic strength (I) of buffer on capacity factors (k′) have been discussed in detail. The capacity factors for TMP increased with increasing pH of both acetate and phosphate buffers. The effects of ionic strength on capacity factors were very substantial and the linear relationship between logk′ and logI was described by the equation logk′=0.3162–0.4420logI with R=–0.9995. The results showed that pH 3.5 acetate buffer (0.05 mol L–1) containing 0.1 mol L–1 sodium chloride and a 1:9 ratio of buffer to methanol were the optimum conditions for separation and determination of TMP. The calibration plot of peak area against concentration was linear with R=0.9979.

Trimethoprim Molecularly imprinted microspheres Chromatography MIP 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Jia-Ping Lai
    • 1
  • Xian-Feng Cao
    • 1
  • Xiu-Ling Wang
    • 2
  • Xi-Wen He
    • 1
  1. 1.Department of Chemistry, Nankai University, Tianjin 300071, P. R. China
  2. 2.College of Life Science, Nankai University, Tianjin 300071, P. R. China

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