Chronic ethanol drinking increases during the luteal menstrual cycle phase in rhesus monkeys: implication of progesterone and related neurosteroids

  • Brandy L. Dozier
  • Cara A. Stull
  • Erich J. Baker
  • Matthew M. Ford
  • Jeremiah P. Jensen
  • Deborah A. Finn
  • Kathleen A. GrantEmail author
Original Investigation



Sporadic reports of alcohol consumption being linked to menstrual cycle phase highlight the need to consider hormonally characterized menstrual cycle phase in understanding the sex-specific effects of risk for alcohol drinking in women.


We investigated the association between menstrual cycle phase, characterized by circulating progesterone and menses, with accurate daily alcohol intakes in rhesus monkeys, and the contribution of progesterone derived neuroactive steroids to cycle-related alcohol drinking.


Menses (daily) and progesterone (2–3×/week) were obtained in female monkeys (n = 8, 5 ethanol, 3 control) for 12–18 months. Ethanol monkeys were then induced to drink ethanol (4% w/v; 3 months) and given 22 h/day access to ethanol and water for approximately 1 year. In selected cycles, a panel of neuroactive steroids were assayed during follicular and luteal phases from pre-ethanol and ethanol exposure.


There were minimal to no effects of ethanol on menstrual cycle length, progesterone levels, and follicular or luteal phase length. The monkeys drank more ethanol during the luteal phase, compared to the follicular phase, and ethanol intake was highest in the late luteal phase when progesterone declines rapidly. Two neuroactive steroids were higher during the luteal phase versus the follicular phase, and several neuroactive steroids were higher in the pre- vs. post-ethanol drinking menstrual cycles.


This is the first study to show that normal menstrual cycle fluctuations in progesterone, particularly during the late luteal phase, can modulate ethanol intake. Two of 11 neuroactive steroids were selectively associated with the effect of cycle progesterone on ethanol drinking, suggesting possible links to CNS mechanisms of ethanol intake control.


Menstrual cycle Luteal phase Alcohol Female Progesterone Monkey Neurosteroid Self-administration 



The authors would like to thank Kevin Nusser for his expertise in animal training, Christa Helms for statistical analysis, Steve Gonzales for development of drinking panels and data analyses, the Endocrine Technology Support Core at the Oregon National Primate Research Center for assaying serum progesterone, and Aleksandr Salo for statistical and graphic contributions.

Funding information

This study was funded by AA013510, AA13641, AA109431 (KAG and EJB), OD11092, AA012439 (DAF) and facilities and resources at the VA Portland Health Care System (DAF).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Comparative MedicineOregon National Primate Research CenterBeavertonUSA
  2. 2.Department of Computer ScienceBaylor UniversityWacoUSA
  3. 3.Division of NeuroscienceOregon National Primate Research CenterBeavertonUSA
  4. 4.Department of Behavioral NeuroscienceOregon Health & Science UniversityPortlandUSA
  5. 5.Department of ResearchVA Portland Health Care SystemPortlandUSA

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