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Psychopharmacology

, Volume 231, Issue 4, pp 753–764 | Cite as

Vendor differences in alcohol consumption and the contribution of dopamine receptors to Pavlovian-conditioned alcohol-seeking in Long-Evans rats

  • Lindsay M. Sparks
  • Joanna M. Sciascia
  • Ziada Ayorech
  • Nadia Chaudhri
Original Investigation

Abstract

Rationale

Drug-associated environmental stimuli elicit craving in humans and drug-seeking in animals.

Objectives

We tested the hypothesis that Pavlovian-conditioned alcohol-seeking is mediated by dopamine, using rats from two vendors.

Methods

Male, Long–Evans rats (220–240 g) from Charles River (St-Constant, QC, Canada) and Harlan Laboratories (Indianapolis, IN, USA) received 21 sessions of intermittent, 24-h access to ethanol (15 %, v/v) and water in the home-cage. Subsequently, rats were trained to discriminate between one conditioned stimulus (CS+) that was paired with ethanol (0.2 ml per CS+) and a second stimulus (CS−) that was not. Entries into a fluid port where ethanol was delivered were recorded. Next, rats were exposed to a different context where cues and ethanol were withheld. At test, responding to the CS+ and CS− without ethanol was assessed in the second, non-alcohol context. Injections (1 ml/kg; s.c.) of the dopamine D1-receptor antagonist SCH 23390 (0, 3.33, and 10 μg/kg) or dopamine D2-receptor antagonist eticlopride (0, 5, and 10 μg/kg) were administered before test.

Results

Home-cage alcohol consumption was higher in Harlan rats than Charles River rats. At test, saline-treated rats responded more to the alcohol-predictive CS+ than the CS−. While SCH 23390 attenuated CS+ responding in rats from both vendors, eticlopride reduced CS+ responding in Harlan rats only. Subsequently, SCH 23390 but not eticlopride attenuated CS+ responding when the CS+ was again paired with ethanol.

Conclusions

These results indicate important differences in alcohol consumption in Long–Evans rats from different suppliers, and highlight a novel role for dopamine in Pavlovian-conditioned alcohol-seeking.

Keywords

Alcoholism Relapse Reinstatement Craving Dopamine Long–Evans SCH 23390 Eticlopride Context Cues 

Notes

Acknowledgments

The National Institute of Alcohol Abuse and Alcoholism (RO1 AA14925; Patricia H. Janak, PI) funded this research. NC is the recipient of a Chercheurs-Boursiers award from Fonds de recherche du Québec - Santé, and a member of the FRQS-funded Center for Studies in Behavioral Neurobiology/Groupe de recherche en neurobiologie comportementale (CSBN/GRNC). The authors would like to thank Dr. Uri Shalev for comments on the manuscript and Atyeh Heidari for assistance in running the experiments.

Supplementary material

213_2013_3292_MOESM1_ESM.docx (70 kb)
ESM 1 (DOCX 70 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Lindsay M. Sparks
    • 1
  • Joanna M. Sciascia
    • 1
  • Ziada Ayorech
    • 1
  • Nadia Chaudhri
    • 1
  1. 1.Center for Studies in Behavioral Neurobiology/Groupe de Recherche en Neurobiologie Comportementale, Department of PsychologyConcordia UniversityMontrealCanada

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