The active alkaloids of Gelsemium elegans Benth. are potent anxiolytics
An increasing number of herbal products has been introduced to treat anxiety and depression. Gelsemium elegans Benth (G. elegans) is a well-known herbal plant in Asia. Four major alkaloids (gelsemine, koumine, gelsevirine, and gelsenicine) have been isolated from G. elegans. Recently, interest has arisen to investigate the pharmaceutical potential of G. elegans alkaloids in the context of neuropsychopharmacology.
We investigated whether G. elegans alkaloids are capable of producing anxiolytic and antidepressant effects in mouse models. In particular, we examined whether the anxiolytic action of G. elegans alkaloids is due to the agonist effects of glycine receptor in the brain.
Two mouse models (elevated plus-maze and light–dark transition model) were used to examine potential anxiolytic effects. Forced swim test and tail suspension test were used to test the antidepressive action of G. elegans alkaloids. Moreover, we also explored the anxiolytic mechanisms of G. elegans alkaloids by intracerebroventricular administration of strychnine, an antagonist of glycine receptor, in the elevated plus-maze.
Gelsemine, koumine, and gelsevirine, but not gelsenicine, exhibited potent anxiolytic effects in the two anxiety models. None of the four G. elegans alkaloids exerted antidepressant effects in the two depression models. None of G. elegans alkaloids impaired spontaneous motor activities. The intracerebroventricular administration of strychnine significantly antagonized the anxiolytic effects of gelsemine, koumine, and gelsevirine administrated subcutaneously.
Gelsemine, koumine, and gelsevirine could be developed as the treatment of anxiety-related disorders in human patients. Their anxiolytic mechanism may be involved in the agonist action of glycine receptor in the brain.
KeywordsGelsemium elegans alkaloids Anxiety Depression Mice Elevated plus-maze Light–dark transition model Forced swim test Tail suspension test Strychnine Glycine receptor
Artificial cerebrospinal fluid
One-way analysis of variance
Forced swim test
Gamma aminobutyric acid A receptor
- G. elegans
Gelsemium elegans Benth
- G. sempervirens
Gelsemium sempervirens Ait
High-speed counter-current chromatography
Light–dark transition model
Median lethal dose
Least significant difference
Tail suspension test
This work was supported by the National Natural Science Foundation of China (No. 81173046, No. 30973520), the Key Program of Scientific Research of Fujian Medical University (No. ZD009), and the Exploitation Program of Industrial Technology of Fujian Development and Reform Commission of China ( No. 958).
Conflict of interest
All authors have no potential conflicts of interest to declare.
- Clement Y, Joubert C, Kopp C, Lepicard EM, Venault P, Misslin R, Cadot M, Chapouthier G (2007) Anxiety in mice: a principal component analysis study. Neural Plast 2007Google Scholar
- Huang ZY, Liu M, Shen J, Su YP, Xu Y, Yu CX (2010) The anti-chronic pain effect of gelsevirine. Chin Tradit Herb Drugs 41:2034–2037Google Scholar
- Shen J, Su YP, Xu Y, Liu H, Liu M, Yu CX (2009) Isolation and purification of gelsenicine and gelsevirine from Gelsemium elegans by high-speed counter-current chromatography. Chin Tradit Herb Drugs 40:1392–1395Google Scholar
- Venard C, Boujedaini N, Mensah-Nyagan AG, Patte-Mensah C (2009) Comparative analysis of gelsemine and Gelsemium sempervirens activity on neurosteroid allopregnanolone formation in the spinal cord and limbic system. Evid Based Complement Altern Med. doi: 10.1093/ecam/nep083