Effects of acupuncture on stress-induced relapse to cocaine-seeking in rats
Cocaine addiction is associated with high rates of relapse, and stress has been identified as a major risk factor. We have previously demonstrated that acupuncture reduces drug self-administration and dopamine release in the nucleus accumbens (NAc), a brain structure implicated in stress-induced reinstatement of drug-seeking behavior.
This study was conducted to investigate the effects of acupuncture on footshock-induced reinstatement of cocaine-seeking and the expression of c-Fos and the transcription factor cAMP response element-binding protein (CREB) in the NAc, used as markers of neuronal activation in conditions of stress-induced reinstatement to cocaine.
Male Sprague–Dawley rats were trained to self-administer cocaine (1.0 mg/kg) for 14 days, followed by extinction and then footshock stress. Acupuncture was applied at bilateral Shenmen (HT7) points for 1 min after footshock stress.
Results and conclusions
Acute footshock stress reinstated cocaine-seeking behavior and enhanced c-Fos expression and phosphorylated CREB (pCREB) activation in the NAc shell in cocaine pre-exposed rats. On the other hand, acupuncture at HT7, but not at control point (LI5), markedly reduced reinstatement of cocaine-seeking (86.5 % inhibition vs. control value), c-Fos expression (81.7% inhibition), and pCREB activation (79.3% inhibition) in the NAc shell. These results suggest that acupuncture attenuates stress-induced relapse by regulating neuronal activation in the NAc shell.
KeywordsCocaine reinstatement Footshock Stress Acupuncture Nucleus accumbens c-Fos pCREB
This work was supported by grants (K08010 and K11010) from Korea Institute of Oriental Medicine (KIOM), South Korea.
- Schoen AM (2001) Veterinary acupuncture: ancient art to modern medicine, 2nd edn. Mosby, St. LouisGoogle Scholar
- Stux G, Pomeranz B (1998) Basics of acupuncture, 4th edn. Springer, BerlinGoogle Scholar
- Ward SJ, Roberts DC (2007) Microinjection of the delta-opioid receptor selective antagonist naltrindole 5′-isothiocyanate site specifically affects cocaine self-administration in rats responding under a progressive ratio schedule of reinforcement. Behav Brain Res 182:140–4PubMedCrossRefGoogle Scholar