Comment on: “Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice” (Magnani P, Conforti A, Zanolin E, Marzotto M and Bellavite P, Psychopharmacology, 2010)
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In a recent article, “Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related response in mice” (Psychopharmacology 2010, 210: 533–545), Magnani et al. claimed to provide solid evidence that Gelsemium sempervirens centesimal dilutions have anxiolytic-like effects in ICR-CD1 mice. This was based on the finding that, with a nonlinear relationship, the centesimal (C) dilution of G. sempervirens 5 C, 9 C and 30 C, but not 4 C and 7 C, modified mice behaviour in the Light Dark test. The result was not confirmed in the Open Field test.
We want to raise some doubts about the reproducibility of the results and the reliability of protocols used to evaluate mice behaviour.
Reproducibility, the degree of accordance between the results of the same experiment performed independently in the same or different laboratories, is a fundamental prerequisite for acceptance of any experimental evidence. This requirement was not met by the authors, who published another...
KeywordsBuspirone Open Field Test High Dilution Spontaneous Locomotor Activity Conforti
Conflict of interest
The authors declare no conflict of interest.
- Bellavite P, Magnani P, Zanolin E, Conforti A (2009a) Homeopathic doses of Gelsemium sempervirens improve the behavior of mice in response to novel environments. Evidence-Based Complementary and Alternative Medicine 2009 Sep 14 [Epub ahead of print]Google Scholar