Identification of multiple call categories within the rich repertoire of adult rat 50-kHz ultrasonic vocalizations: effects of amphetamine and social context
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50-kHz ultrasonic vocalizations (USVs) emitted by adult rats are heterogeneous; they occur over a wide frequency range, show varying degrees of frequency modulation, and appear to differ in their behavioral significance. However, they have not been extensively categorized.
The main objective of this study was to identify subtypes of 50-kHz USVs emitted by adult rats and to determine how amphetamine (AMPH) or social testing condition affects their relative and absolute production rate and acoustic characteristics. A second objective was to determine the extent of individual differences in call rate, call subtype profile, and acoustic parameters (i.e., duration, bandwidth, and mean peak frequency).
Adult male Long–Evans rats were administered systemic amphetamine (0.25–2 mg/kg, IP) and tested individually or with a cage mate for 20 min. Call categories were defined based on visual inspection of over 20,000 USV spectrograms. Surgical devocalization was performed on a subset of AMPH-tested rats in order to confirm the authenticity of call subtypes.
Fourteen categories of 50-kHz USVs were recognized. Call subtypes were differentially affected by social context, AMPH dose, and time within session. In contrast, the acoustic characteristics of call subtypes were notably stable. Marked and stable inter-individual differences occurred with respect to overall 50-kHz call rate, acoustic parameters, and call profile.
The present findings, obtained under saline and amphetamine test conditions, provide the first detailed classification of adult rat 50-kHz USVs. Consideration of 50-kHz USV subtypes may advance our understanding of inter-rat communication and affective state.
KeywordsUltrasonic vocalizations Amphetamine Reward Frequency-modulated Trill Dose–response Individual differences
This study was supported by a Natural Science and Engineering Research Council of Canada discovery grant (155055, to P.B.S.C), a Canadian Institutes of Health Research of Canada operating grant (MOP-10516, to P.B.S.C), and a Master’s Research Scholarship from the Fonds Québécois de Recherche sur la Nature et les Technologies (to J.M.W). We would like to thank Diala Chehayeb for valuable discussions and Laura Desrochers for help with the reliability testing. The authors have no financial relationship with the organizations that sponsored this research. Declaration: all experiments comply with the current laws of Canada.
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