Cocaine-induced behavioral sensitization in preweanling and adult rats: effects of a single drug–environment pairing
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Adult rats typically exhibit more robust behavioral sensitization than do preweanling rats. A possible explanation for this age-dependent difference is that environmental context may have relatively less impact on the psychostimulant-induced behaviors of preweanling rats.
The purpose of this study was to assess the importance of environmental context for the development of cocaine-induced sensitization in preweanling and adult rats.
Materials and methods
On postnatal day (PD) 19 or PD 79, rats in the context-dependent condition were injected with 30 mg/kg cocaine immediately before being placed in a novel test chamber for 30 min. The same rats were then injected with saline 30 min after being returned to the home cage. Rats in the context-independent condition were injected with saline before being placed in the novel chamber and cocaine in the home age. Control rats were injected with saline at both time points. One day later, adult and preweanling rats were challenged with saline or 10 mg/kg cocaine (experiment 1), or preweanling rats were challenged with 5, 20, or 30 mg/kg cocaine (experiment 2). After being injected, rats were placed in the test chamber, and behavior was measured for 60 min.
Adult rats showed context-dependent locomotor sensitization and conditioned activity, with females exhibiting more locomotor activity than males. Preweanling rats did not exhibit conditioned activity, but they showed robust context-dependent and context-independent sensitization when challenged with 10–30 mg/kg cocaine.
Context did not influence the expression of behavioral sensitization in preweanling rats, suggesting that deficits in associative or memory processes may be responsible for age-dependent differences in behavioral sensitization and conditioned activity.
KeywordsBehavioral sensitization Conditioned activity Cocaine Preweanling rats Ontogeny Associative processes
- National Research Council (2003) Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research. National Academy Press, Washington, DCGoogle Scholar
- Ostrander MM, Badiani A, Day HE, Norton CS, Watson SJ, Akil H, Robinson TE (2003) Environmental context and drug history modulate amphetamine-induced c-fos mRNA expression in the basal ganglia, central extended amygdala, and associated limbic forebrain. Neuroscience 120:551–571PubMedCrossRefGoogle Scholar
- Robinson TE, Becker JB, Presty SK (1982) Long-term facilitation of amphetamine-induced rotational behavior and striatal dopamine release produced by a single exposure to amphetamine: sex differences. Brain Res 571:330–337Google Scholar
- Stewart J, Vezina P (1988) Conditioning and sensitization. In: Kalivas PW, Barnes CD (eds) Sensitization in the nervous system. Telford Press, Caldwell, NJ, pp 207–224Google Scholar