Medial prefrontal cortical alpha1 adrenoreceptor modulation of the nucleus accumbens dopamine response to stress in Long–Evans rats
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The medial prefrontal cortex (PFC) receives stress-sensitive dopamine (DA) and noradrenergic (NE) projections from the ventral tegmental area and locus coeruleus, respectively, and evidence from various sources point to a complex functional interaction between these two systems. Stress will also stimulate DA transmission in the nucleus accumbens (NAcc), and our previous work has shown that this response is under the indirect inhibitory control of a DA-sensitive mechanism in PFC.
We examined the possibility that the NAcc DA stress response is also modulated by prefrontal cortical NE.
Materials and methods
We used voltammetry to study in freely behaving rats the effects of local applications of alpha1 (benoxathian 0.1, 1, 10 nmol), alpha2 (SKF86466), and beta1/2 (alprenolol) receptor selective antagonists into the PFC on the NAcc DA response to tail-pinch stress.
The NAcc DA stress response was dose-dependently inhibited by local PFC blockade of alpha1 receptors. Additional tests revealed, however, that the DA stress response in NAcc is unaffected after local alpha1 receptor activation with cirazoline. Furthermore, at equivalent doses, neither alpha2 nor beta1/2 receptor blockade significantly affected the NAcc DA stress response.
These data indicate that stress-induced activation of subcortical DA transmission is modulated by the NE input to PFC acting at alpha1 receptors. They suggest that, under normal circumstances, this system exerts a facilitatory or enabling influence on the NAcc DA stress response.
KeywordsVoltammetry Benoxathian SKF86466 Cirazoline Alprenolol
This research was funded by a Canadian Institutes of Health Research grant to A.G.
- Doherty MD, Gratton A (2007) Differential involvement of ventral tegmental GABAA and GABAB receptors in the regulation of the nucleus accumbens dopamine response to stress. Brain Res (in press)Google Scholar
- Gobert A, Rivet JM, Audinot V, Newman-Tancredi A, Cistarelli L, Millan MJ (1998) Simultaneous quantification of serotonin, dopamine and noreadrenaline levels in single frontal cortex dialysates of freely-moving rats reveals complex pattern of reciprocal auto- and heteroreceptor-mediated control of release. Neurosci 84:413–429CrossRefGoogle Scholar
- Kiyatkin EA (1988) Functional properties of presumed dopamine-containing and other ventral tegmental area neurons in conscious rats. Int J Neurosci 42:21–43Google Scholar
- Morari M, O’Connor WT, Ungerstedt U, Bianchi C, Fuxe K (1996) Functional neuroanatomy of the nigrostriatal and striatonigral pathways as studied with dual probe microdialysis in the awake rat—II. Evidence for striatal N-Methyl-d-Aspartate receptor regulation of striatonigral GABAergic transmission and motor function. Neurosci 72:89–97CrossRefGoogle Scholar
- Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic, New YorkGoogle Scholar
- Smythe JW, Gratton A (1994) Effects of stress on ventral tegmental unit activity in freely-behaving rat. Soc Neurosci Abstr 20:821Google Scholar