, Volume 185, Issue 1, pp 93–103 | Cite as

Effect of creatine supplementation and sleep deprivation, with mild exercise, on cognitive and psychomotor performance, mood state, and plasma concentrations of catecholamines and cortisol

  • T. McMorrisEmail author
  • R. C. Harris
  • J. Swain
  • J. Corbett
  • K. Collard
  • R. J. Dyson
  • L. Dye
  • C. Hodgson
  • N. Draper
Original Investigation



Sleep deprivation has a negative effect on cognitive and psychomotor performance and mood state, partially due to decreases in creatine levels in the brain. Therefore, creatine supplementation should lessen the negative effects of sleep deprivation.


The objective of this study was to examine the effect of creatine supplementation and sleep deprivation, with mild exercise, on cognitive and psychomotor performance, mood state, and plasma concentrations of catecholamines and cortisol.


Subjects were divided into a creatine group (n=10) and a placebo group (n=9). They took 5 g of creatine monohydrate or a placebo, dependent on their group, four times a time a day for 7 days, immediately prior to the experiment. The study was double blind. Subjects undertook tests of random movement generation (RMG), verbal and spatial recall, choice reaction time, static balance and mood state pre-test (0 h), after 6, 12 and 24 h of sleep deprivation, with intermittent exercise. They were tested for plasma concentrations of catecholamines and cortisol at 0 and 24 h.


At 24 h, the creatine group demonstrated significantly less change in performance from 0 h (Δ) in RMG, choice reaction time, balance and mood state. There were no significant differences between groups in plasma concentrations of catecholamines and cortisol. Norepinephrine and dopamine concentrations were significantly higher at 24 h than 0 h, but cortisol were lower.


Following 24-h sleep deprivation, creatine supplementation had a positive effect on mood state and tasks that place a heavy stress on the prefrontal cortex.


Stress Working memory Prefrontal cortex Choice reaction time Balance 



This research was funded by the Howard Foundation of Cambridge, to whom the authors are very grateful.


  1. AbuRaz S, Millership J, Heaney L, McElnay J (2003) Simple liquid chromatography method for the rapid simultaneous determination of prednisolone and cortisol in plasma and urine using hydrophilic lipophilic balanced solid phase extraction cartridges. J Chromatogr B 798:193–201CrossRefGoogle Scholar
  2. Annoni JM, Pegna AJ (1997) Random motor generation in a finger tapping task: influence of spatial contingency and of cortical and subcortical hemispheric brain lesions. J Neurol Neurosurg Psychiatry 63:654–659PubMedCrossRefGoogle Scholar
  3. Baddeley AD (1986) Working memory. Oxford University Press, New YorkGoogle Scholar
  4. Baddeley AD, Emslie H, Kolodny J, Duncan J (1998) Random generation and the central executive of working memory. Q J Exp Psychol A 51:819–852PubMedCrossRefGoogle Scholar
  5. Bares M, Rektor I, Kanovsky P, Streitova H (2003) Cortical and sub-cortical distribution of middle and long latency auditory and visual evoked potentials in a cognitive (CNV) paradigm. Clin Neurophysiol 114:2447–2460PubMedCrossRefGoogle Scholar
  6. Braun AR, Balkin TJ, Wesenten NJ, Carson RE, Varga M, Baldwin P, Selbie S, Belenky G, Herscovitch P (1997) Regional cerebral blood flow throughout the sleep-wake cycle-An (H20)-0-15 PET study. Brain 120:1173–1197PubMedCrossRefGoogle Scholar
  7. Brugger P (1997) Variables that influence the generation of random sequences: an update. Percept Mot Skills 84:627–661PubMedGoogle Scholar
  8. Cohen J (1992) A power primer. Psychol Bull 112:155–159CrossRefPubMedGoogle Scholar
  9. Dechent P, Pouwels PJW, Wilken B, Hanefeld F, Frahm J (1999) Increase of total creatine in human brain after oral supplementation of creatine-monohydrate. Regul Integr Comp Physiol 46:R698-R704Google Scholar
  10. Deiber MP, Passingham RE, Colebatch JG, Friston KJ, Nixon PD, Frackowiak RSJ (1991) Cortical areas and selection of movement—a study with positron emission tomography. Exp Brain Res 84:393–402PubMedCrossRefGoogle Scholar
  11. Dietrich A, Sparling PB (2004) Endurance exercise selectively impairs prefrontal-dependent cognition. Brain Cogn 55:516–524PubMedCrossRefGoogle Scholar
  12. Drevets WC, Burton H, Simpson JR, Videen TO, Snyder AZ, Raichle ME (1995) Cerebral blood flow decreases in primary somatosensory cortex during anticipation of somatosensory stimulation. Nature 373 249–252PubMedCrossRefGoogle Scholar
  13. Evans FJ (1978) Monitoring attention deployment by random number generation: an index to measure subjective randomness. Bull Psychon Soc 12:35–38Google Scholar
  14. Eysenck MW, Calvo MG (1992) Anxiety and performance—the processing efficiency theory. Cogn Emot 6:409–434CrossRefGoogle Scholar
  15. Frith CD, Frison K, Liddle PF, Frackowiak RSJ (1991) Willed action and the prefrontal cortex in man—a study with PET. Proc R Soc Lond B 244:241–246CrossRefGoogle Scholar
  16. Greenhaff PL, Casey A, Short AH, Harris R, Soderlund K, Hultman E (1993) Influence of oral creatine supplementation of muscle torque during repeated bouts of maximal voluntary exercise in man. Clin Sci 84:567–571Google Scholar
  17. Grove J, Prapavessis H (1992) Preliminary evidence for the reliability and validity of an abbreviated profile of mood states. Int J Sport Psychol 23:93–109Google Scholar
  18. Harris RC, Soderlund K, Hultman E (1992) Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation. Clin Sci 83:367–374PubMedGoogle Scholar
  19. He HB, Stein CM, Christman B, Wood AJJ (1997) Determination of catecholamines in sheep plasma by high-performance liquid chromatography with electrochemical detection: comparison of deoxyepinephrine and 3,4-dihydroxybenzylamine as internal standard. J Chromatogr B 701:115–119CrossRefGoogle Scholar
  20. Heuer H, Kohlisch O, Klein W (2005) The effects of total sleep deprivation on generation of random sequences of key-presses, numbers and nouns. Q J Exp Psyhcol A 58:273–307Google Scholar
  21. Hoffman JR, Maresh CM, Armstrong LE, Gabaree CL, Bergeron MF, Kenefick RW et al (1994) Effects of hydration state on plasma testosterone, cortisol and catecholamine concentrations before and during mild exercise at elevated temperature. Eur J Appl Physiol 69:294–300CrossRefGoogle Scholar
  22. Jennings JR, Monk TH, van der Molen MW (2003) Sleep deprivation influences some but not all processes of supervisory attention. Psychol Sci 14:473–479PubMedCrossRefGoogle Scholar
  23. Kim D-J, Lee H-P, Kim MS, Park Y-J, Go H-J, Kim K-S et al (2001) The effect of total sleep deprivation on cognitive functions in normal adult male subjects. Int J Neurosci 109:127–137PubMedCrossRefGoogle Scholar
  24. McMorris T, Sproule J, Childs R, Draper S, Sexsmith JR (2000) The measurement of plasma lactate and catecholamine thresholds: a comparison of methods. Eur J Appl Physiol 82:262–267PubMedCrossRefGoogle Scholar
  25. McNair DM, Loer M, Droppleman LF (1971) Manual for the profile of mood states. Educational and Industrial Testing Sciences, San Diego, CAGoogle Scholar
  26. Melin B, Jimenez C, Savourey G, Bittel J, Cottet-Emard JM, Pequinot JM (1997) Effect of hydration state on hormonal and renal responses during moderate exercise in the heat. Eur J Appl Physiol 76:320–327CrossRefGoogle Scholar
  27. Meerlo P, Koehl M, van der Borght K, Turek FW (2002) Sleep restriction alters the hypothalamic–pituitary–adrenal response to stress. J Neuroendocrinol 14:397–402PubMedCrossRefGoogle Scholar
  28. Meeusen R, Piacentini MF (2003) Exercise, fatigue, neurotransmission and the influence of the neuroendocrine axis. Adv Exp Med Biol 57:521–525Google Scholar
  29. Millan MJ (2004) The role of monoamines in the actions of established and “novel” antidepressant agents: a critical review. Eur J Pharmacol 500:371–384PubMedCrossRefGoogle Scholar
  30. Neuringer A (1986) Can people behave randomly—the role of feedback. J Exp Psychol Gen 115:62–75CrossRefGoogle Scholar
  31. Opstad PK (1991) Alterations in the morning plasma levels of hormones and the endocrine responses to bicycle exercise during prolonged strain. The significance of energy and sleep deprivation. Acta Endocrinol (Copenhagen) 125:14–22Google Scholar
  32. Peyrin L, Pequinot JM, Lacour JR, Fourcade J (1987) Relationships between catecholamine or 3-methoxy 4-hydroxy phenylglycol changes and mental performance under submaximal exercise in man. Psychopharmacology 93:188–192PubMedCrossRefGoogle Scholar
  33. Rae C, Digney AL, McEwan SR, Bates TC (2003) Oral creatine monohydrate supplementation improves brain performance: a double-blind, placebo-controlled, cross-over trial. Proc R Soc Lond B 270:2147–2150CrossRefGoogle Scholar
  34. Smith EE, Jonides J (1999) Neuroscience—storage and executive processes in the frontal lobes. Science 283:1657–1661PubMedCrossRefGoogle Scholar
  35. Towse JN, Neil D (1998) Analyzing human random generation behavior: a review of methods used and a computer program for describing performance. Behav Res Methods Instrum Comput 30:583–591Google Scholar
  36. Towse JN, Valentine JD (1997) Random generation of numbers: a search for underlying processes. Eur J Cogn Psychol 9:381–400CrossRefGoogle Scholar
  37. Vedhara K, Hyde J, Gilchrist ID, Tytherleigh M, Plummer S (2000) Acute stress, memory, attention and cortisol. Psychoneuroendocrinology 25:535–549PubMedCrossRefGoogle Scholar
  38. von Treuer K, Norman TR, Armstong SM (1996) Overnight human plasma melatonin, cortisol, prolactin, TSH, under conditions of normal sleep, sleep deprivation, and sleep recovery. J Pineal Res 20:7–14CrossRefGoogle Scholar
  39. Watanabe A, Kato N, Kato T (2002) Effect of creatine on mental fatigue and cerebral hemoglobin oxygenation. Neurosci Res 42:279–285PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • T. McMorris
    • 1
    Email author
  • R. C. Harris
    • 1
  • J. Swain
    • 1
  • J. Corbett
    • 1
  • K. Collard
    • 2
  • R. J. Dyson
    • 1
  • L. Dye
    • 3
  • C. Hodgson
    • 4
  • N. Draper
    • 4
  1. 1.Centre for Sports Science and MedicineUniversity College ChichesterWest SussexUK
  2. 2.School of Health ProfessionsUniversity of PlymouthExeterUK
  3. 3.Institute of Psychological SciencesUniversity of LeedsLeedsUK
  4. 4.Centre for Research in Adventure ScienceUniversity College ChichesterWest SussexUK

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