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Psychopharmacology

, Volume 184, Issue 1, pp 26–35 | Cite as

The relationship of in vivo central CB1 receptor occupancy to changes in cortical monoamine release and feeding elicited by CB1 receptor antagonists in rats

  • Anne B. Need
  • Richard J. Davis
  • Jesline T. Alexander-Chacko
  • Brian Eastwood
  • Eyassu Chernet
  • Lee A. Phebus
  • Dana K. Sindelar
  • George G. NomikosEmail author
Original Investigation

Abstract

Rationale

Cannabinoid type 1 (CB1) receptor antagonists are reportedly effective in reducing food intake both preclinically and clinically. This may be due in part to their effects on monoamine release in the brain. The level of central CB1 receptor occupancy underlying these neurobiological effects is unclear.

Objectives

We explored the relationship between in vivo CB1 receptor occupancy in the frontal cortex and changes in both monoamine release in the medial prefrontal cortex (mPFC) and feeding behavior in rats in response to two orally administered CB1 receptor antagonists presently in clinical trials, SR141716A (rimonabant) and SLV319.

Methods

CB1 receptor occupancy was measured using [3H] SR141716A, and these occupancies were related to potencies to mediate increases in dopamine (DA) and norepinephrine (NE) release measured with microdialysis and decreases in consumption of a highly palatable diet (HP).

Results

High receptor occupancy levels (>65%) were required to detect increases in monoamine release that were achieved with 3 and 10 mg/kg of SR141716A and 10 mg/kg of SLV319 for DA and 10 mg/kg of SR141716A for NE. Decreases in HP consumption were seen at occupancies higher than 65% for SR141716A that were achieved with 3 and 10 mg/kg. In contrast, decreases in HP consumption were seen at relatively low CB1 receptor occupancies (11%) for SLV319.

Conclusions

The occupancy method described here is an effective tool for interrelating central CB1 receptor occupancy with neurobiological actions of CB1 receptor antagonists and for furthering our understanding of the role of CB1 receptors in central nervous system physiology and pathology.

Keywords

SR141716A SLV319 [3H]SR141716A Dopamine Norepinephrine Microdialysis Receptor occupancy Feeding Cannabinoid In vivo binding 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Anne B. Need
    • 1
  • Richard J. Davis
    • 1
  • Jesline T. Alexander-Chacko
    • 1
  • Brian Eastwood
    • 1
  • Eyassu Chernet
    • 1
  • Lee A. Phebus
    • 1
  • Dana K. Sindelar
    • 1
  • George G. Nomikos
    • 1
    Email author
  1. 1.Lilly Research LaboratoriesLilly Corporate CenterIndianapolisUSA

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