, Volume 179, Issue 3, pp 551–558 | Cite as

Effects of the plant-derived hallucinogen salvinorin A on basal dopamine levels in the caudate putamen and in a conditioned place aversion assay in mice: agonist actions at kappa opioid receptors

  • Yong ZhangEmail author
  • Eduardo R. Butelman
  • Stefan D. Schlussman
  • Ann Ho
  • Mary Jeanne Kreek
Original Investigation



Salvinorin A is a naturally occurring hallucinogen derived from the plant Salvia divinorum. Salvinorin A is also a potent and selective kappa opioid receptor agonist in vitro. It has been shown that kappa agonists decrease dopamine levels in the caudate putamen and nucleus accumbens and cause conditioned place aversion in rodents.


To study the effects of salvinorin A on basal dopamine levels in the caudate putamen and nucleus accumbens, and to determine whether salvinorin A induces conditioned place preference or aversion and changes in locomotor activity in the mouse.


In the first experiment, changes in dopamine levels in these brain regions after administration of salvinorin A were measured with in vivo microdialysis. In the second experiment, we examined whether salvinorin A led to conditioned place preference or aversion, and changes in locomotor activity during conditioning sessions.


The higher doses of salvinorin A studied (1.0 mg/kg and 3.2 mg/kg, i.p.) significantly decreased dopamine levels in the caudate putamen, but not in the nucleus accumbens, and this effect was completely blocked by pre-injection with 10 mg/kg of the kappa opioid receptor antagonist nor-binaltorphimine. The same doses of salvinorin A caused conditioned place aversion and decreased locomotor activity.


The inhibitory effect of salvinorin A on striatal dopamine levels may contribute to its induction of conditioned place aversion and decreases in locomotion in mice. These findings are consistent with the in vitro characterization of salvinorin A as a kappa opioid receptor agonist. It is of interest that a compound such as salvinorin A, that lowers striatal dopamine levels and leads to conditioned place aversion in rodents, is self-administered by humans under certain conditions.


Salvinorin A Dopamine Microdialysis Mice Conditioned place aversion 



We thank Susan Russo for her careful reading of the manuscript. This work was supported by the National Institutes of Health-National Institute on Drug Abuse Research Center Grant P60-DA05130 and National Institute on Drug Abuse Research Scientist Award K05-DA00049 to Dr. Mary Jeanne Kreek.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Yong Zhang
    • 1
    Email author
  • Eduardo R. Butelman
    • 1
  • Stefan D. Schlussman
    • 1
  • Ann Ho
    • 1
  • Mary Jeanne Kreek
    • 1
  1. 1.The Laboratory of the Biology of Addictive DiseasesThe Rockefeller UniversityNew YorkUSA

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