Psychopharmacology

, Volume 166, Issue 4, pp 373–382 | Cite as

Antidepressant-like effects in various mice strains in the forced swimming test

  • Denis Joseph Paul David
  • Caroline E. Renard
  • Pascale Jolliet
  • Martine Hascoët
  • Michel Bourin
Original Investigation

Abstract

Rationale

Strain differences in mice have been reported in response to drugs in the mouse forced swimming test (FST), even if few antidepressants were examined.

Objectives

The aim of the present study was to investigate the influence of genetic factors, using five antidepressants (imipramine, desipramine, citalopram, paroxetine and bupropion) in the mouse FST, in outbred strains (Swiss, NMRI) and inbred strains (DBA/2, C57BL/6J Rj). Moreover, whole brain levels of dopamine (DA), noradrenaline (NA), serotonin (5-HT) in vehicle treated animals, which were or were not subjected to the FST, were measured by HPLC analysis in an attempt to explain behavioural differences.

Methods

For each antidepressant, a dose range (1–16 mg/kg) was tested in the locomotor apparatus and only non-psychostimulant doses were then tested in the FST in order to detect antidepressant-like activity.

Results

No baseline differences among Swiss, NMRI, DBA/2 and C57BL/6J Rj strains were observed in our experiments, allowing the comparison of different antidepressants in each strain. Imipramine (16 mg/kg), desipramine, citalopram (4–16 mg/kg) and paroxetine (8 and 16 mg/kg) treatment decreased the immobility time in the Swiss strain and the size of the effect reached more than 20% for each of these antidepressants. C57BL/6J Rj was the only strain sensitive to bupropion (2 and 4 mg/kg). In the NMRI strain, only paroxetine treatment decreased the immobility time (16 mg/kg).

Conclusion

Our study showed that drug sensitivity is genotype dependent. FST results have shown that Swiss mice are the most sensitive strain to detect 5-HT and/or NA treatment. The use of DBA/2 inbred mice may be limited, as an absence of antidepressant-like response was observed in the FST. The lack of sensitivity to antidepressant treatment in DBA/2 strains could be due to high DA, NA and 5-HT whole brain concentrations.

Keywords

Antidepressant Force swim test Mouse strain Neurotransmitters Drug sensitivity 

References

  1. Ascher JA, Cole JO, Colin JN, Feighner JP, Ferris RM, Fibiger, HC, Golden RN, Martin P, Potter WZ, Richelson E, Sulser F (1995) Bupropion: a review of its mechanism of antidepressant activity. J Clin Psychiatry 56:395–401PubMedGoogle Scholar
  2. Bai F, Li X, Clay M, Lindstrom T, Skolnick P (2001) Intra- and interstrain differences in models of "behavioral despair". Pharmacol Biochem Behav 70:187–192Google Scholar
  3. Baker GB, Coutts RT, Rao TS (1987) Neuropharmacological and neurochemical properties of N-(cyanoethyl)-beta-phenylethylamine, a prodrug of beta-phenylamine. Br J Pharmacol 92:243–256PubMedGoogle Scholar
  4. Boissier JR, Simon P (1965) Action de la caféine sur la motilité spontanée de la souris. Arch Int Pharmacodyn 158:212–221Google Scholar
  5. Borsini F (1995) Role of the serotonergic system in the forced swimming test. Neurosci Biobehav Rev 19:377–395PubMedGoogle Scholar
  6. Borsini F, Meli A (1988) Is the forced swimming test a suitable model for revealing antidepressant activity? Psychopharmacology 94:147–160PubMedGoogle Scholar
  7. Bourin M, Colombel MC, Redrobe JP, Nizart J, Hascoët M, Baker GB (1998) Evaluation of efficacies of different classes of antidepressants in the forced swimming test in mice at different ages. Prog Neuropsychopharmacol Biol Psychiatry 22:343–351CrossRefPubMedGoogle Scholar
  8. Crawley JN, Belknap JK, Collins A, Crabbe JC, Frankel W, Henderson N, Hitzemann RJ, Maxson N, Miner LL, Silva AJ, Wehner JM, Wynshaw-Boris A, Paylor R (1997) Behavioural phenotypes of inbred mouse strains: implications and recommendations for molecular studies. Psychopharmacology 132:107–124PubMedGoogle Scholar
  9. Crusio WE, Schwegler H, van Abeelen JH (1991) Behavioural and neuroanatomical divergence between two sublines of C57BL/6J inbred mice. Behav Brain Res 42:93–97PubMedGoogle Scholar
  10. Cryan JF, Dalvi A, Jin SH, Hirsch R, Lucki I, Thomas SA (2001) Use of dopamine-β-hydroxylase-deficient mice to determine the role of norepinephrine in the mechanism of action of antidepressant drugs. J Pharmacol Exp Ther 298:651–657PubMedGoogle Scholar
  11. Dalvi A, Lucki I (1999) Murine models of depression. Psychopharmacology 147:14–16Google Scholar
  12. David DJP, Bourin M, Hascoët M, Colombel MC, Baker GB, Jolliet P (2001a) Comparison of antidepressant activity in 4- and 40- week-old male mice in the forced swimming test: involvement of 5-HT1A and 5-HT1B receptors in old mice. Psychopharmacology 153:443–449CrossRefPubMedGoogle Scholar
  13. David DJP, Nic Dhonnchadha BÁ, Jolliet P, Hascoët M, Bourin M (2001b) The use of animal models in defining antidepressant response. Brain Pharmacol 1:11–35Google Scholar
  14. Davis WM, King WT, Babbini M (1967) Placebo effect of saline on locomotor activity in several strains of mice. J Pharm Sci 56:1347–1349PubMedGoogle Scholar
  15. Duman RS, Heninger GR, Nestler EJ (1997) A molecular and cellular theory of depression. Arch Gen Psychiatry 54:597–606PubMedGoogle Scholar
  16. Gardier AM, Trillat AC, Malagié I, David D, Hascoët M, Colombel MC, Jolliet P, Jacquot C, Hen R, Bourin M (2001) Récepteurs 5-HT1B de la sérotonine et effets antidépresseurs des inhibiteurs de recapture sélectifs de la sérotonine. CR Acad Sci 324:433–441CrossRefGoogle Scholar
  17. Hilakivi LA, Lister RG (1990) Correlations between behaviour of mice in Porsolt's swim test and in tests of anxiety, locomotion and exploration. Behav Neural Biol 53:153–159PubMedGoogle Scholar
  18. Hwang BH, Kunkler PE, Tarricone BJ, Hingtegen JN, Nurnberger JL Jr (1999) Stress-induced changes of norepinephrine uptakes sites in the locus coeruleus of C57BL/6J and DBA/2J mice: a quantitative autoradiographic study using [3H]-tomoxetine. Neuroscience Lett 265:151–154CrossRefGoogle Scholar
  19. Katkov YA, Otmakhova NA, Gurevich EV, Nesterova IV, Bobkova NV (1994) Antidepressants suppress bulbectomy-induced augmentation of voluntary Alcohol Consumption in C57BL/6J but not in DBA/2 mice. Physiol Behav 56:501–509PubMedGoogle Scholar
  20. Logue SF, Owen EH, Rasmussen DL, Wehner JM (1997) Assessment of locomotor activity, acoustic and tactile startle, and prepulse inhibition of startle in inbred mouse strains and F1 hybrids: implications of genetic background for single gene and quantitative trait loci analyses. Neuroscience 80:1075–1086CrossRefPubMedGoogle Scholar
  21. Lucki I, Dalvi A, Mayorga AJ (2001) Sensitivity to the effects of pharmacologically selective antidepressants in different strains of mice. Psychopharmacology 155:315–322PubMedGoogle Scholar
  22. Malinge M, Bourin, Colombel MC, Larousse C (1988) Additive effects of clonidine and antidepressant drugs in the mouse forced swimming test. Psychopharmacology 96:104–109PubMedGoogle Scholar
  23. Martin P, Massol J, Colin JN, Lacomblez L, Puech A (1990) Antidepressant profile of bupropion and three metabolites in mice. Pharmacopsychiatry 23:187–194PubMedGoogle Scholar
  24. Nikulina EM, Skrinskaya JA, Popova NK (1991) Role of genotype and dopamine receptors in behaviour of inbred mice in a forced swimming test. Psychopharmacology 105:525–529PubMedGoogle Scholar
  25. Otmakhova NA, Gurevich EV, Katkov YA, Nesterova IV, Bobkova NV (1992) Dissociation of multiple behavioural effects between olfactory bulbectomized C57BL/6J and DBA/2 mice. Physiol Behav 52:441–448PubMedGoogle Scholar
  26. Porsolt RD, Bertin A, Jalfre M (1977) Behavioural despair in mice: a primary screening test for antidepressants. Arch Int Pharmacodyn 229:327–336PubMedGoogle Scholar
  27. Porsolt RD, Anton G, Blavet N, Jalfre M (1978) Behavioral despair in rats: a new model sensitive to antidepressant treatment. Eur J Pharmacol 47:379–391PubMedGoogle Scholar
  28. Ravard S, Carnoy P, Hervé D, Tassin JP, Thiebot MH, Soubrié P (1990) Involvement of prefrontal dopamine neurones in behavioural blockade induced by controllable vs uncontrollable negative events in rats. Behav Brain Res 37:9–18PubMedGoogle Scholar
  29. Redrobe JP, Bourin M (1997) Partial role of 5-HT2 and 5-HT3 receptors in the activity of antidepressants in the mouse forced swimming test. Eur J Pharmacol 325:129–135PubMedGoogle Scholar
  30. Redrobe JP, Bourin M (1999) Augmentation of antidepressant pharmacotherapy: a preclinical approach using the mouse forced swimming test. CNS Spectrums 4:73–81Google Scholar
  31. Redrobe JP, MacSweeney CP, Bourin M (1996) The role of 5-HT1A and 5-HT1B receptors in antidepressant drugs actions in the mouse forced swimming test. Eur J Pharmacol 318:213–220PubMedGoogle Scholar
  32. Redrobe JP, Bourin M, Colombel MC, Baker GB (1998) Psychopharmacological profile of the selective serotonin reuptake inhibitor, paroxetine: implication of noradrenergic and serotonergic mechanisms. J Psychopharmacol 12:348–355PubMedGoogle Scholar
  33. Renard CE, Dailly E, David DJP, Hascoët M, Bourin M (2003) Neurochemical changes following the mouse forced swimming test but not the tail suspension test. Fund Clin Pharmacol (in press)Google Scholar
  34. Rogers DC, Jones DNC, Nelson PR, Jones CM, Quilter CA, Robinson TL, Hagan JJ (1999) Use of SHIRPA and discriminant analysis to characterize marked differences in the behavioural phentotype of six inbred mouse strains. Behav Brain Res 105:207–217PubMedGoogle Scholar
  35. Sánchez C, Hyttel J (1999) Comparison of effects of antidepressants and their metabolites on reuptake of biogenic amines and on receptor binding. Cell Mol Neurobiol 19:467–488Google Scholar
  36. Sánchez C, Meier (1997) Behavioural profiles of SSRIs in animal models of depression, anxiety and aggression. Psychopharmacology 129:197–205PubMedGoogle Scholar
  37. Tadano T, Abe Y, Morikawa Y, Hozumi M, Nakagawasai O, Tan-No K, Kisara K (1997) Relationship between learning behaviour and genetic factor on immobility shown during forced swimming test. Nihon Shinkei Seishin Yakurigaku Zasshi 3:129–135Google Scholar
  38. Trullas R, Skolnick P (1993) Differences in fear motivated behaviours among inbred mouse strains. Psychopharmacology 111:323–331PubMedGoogle Scholar
  39. Trullas R, Barrington J, Skolnick P (1989) Genetic differences in a tail suspension test for evaluating antidepressant activity. Psychopharmacology 99:287–288Google Scholar
  40. Van der Heyden JA, Zethof TJ, Olivier B (1987) Stress-induced hyperthermia in singly housed mice. Physiol Behav 62:463–470Google Scholar
  41. Van Gaalen MM, Steckler T (2000) Behavioural analysis of four mouse strains in an anxiety test. Behav Brain Res 115:95–106PubMedGoogle Scholar
  42. Võikar V, Kõks S, Vasar E, Rauvala H (2001) Strain and gender differences in the behaviour of mouse lines commonly used in transgenic studies. Physiol Behav 72:271–281PubMedGoogle Scholar
  43. Yadid G, Overstreeet DH, Zangen A (2001) Limbic dopaminergic adaptation to a stressful stimulus in a rat model of depression. Brain Res 896:43–47CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Denis Joseph Paul David
    • 1
  • Caroline E. Renard
    • 1
  • Pascale Jolliet
    • 1
  • Martine Hascoët
    • 1
  • Michel Bourin
    • 1
  1. 1.EA 3256 Neurobiologie de l'anxiété et de la dépressionFaculté de MédecineNantes Cedex 01France

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