Bromelain reversed electrolyte imbalance in the chronically constricted sciatic nerve of Wistar rats

  • Ahmed Olalekan BakareEmail author
  • Bamidele Victor Owoyele
Original Article


Derangement of electrolyte in the sensory nervous system has been attributed to the development and maintenance of hyperalgesic and allodynic symptoms in painful neuropathy. This study investigated the effect of bromelain on electrolyte imbalance in chronically constricted sciatic nerve of rats (a model of neuropathic pain). Forty Wistar rats, divided into five groups of eight animals each were used for this study. von Frey filaments, tail immersion and acetone spray tests were used to assessed allodynic and thermal hyperalgesic symptoms in the Wistar rats. Sodium ion (Na+), potassium ion (K+), calcium ion (Ca2+) and chloride ion (Cl) concentrations as well as sodium-potassium and calcium electrogenic pump (Na-K ATPase and Ca ATPase, respectively) activities were estimated using spectrophotometry techniques. Bromelain significantly (p < 0.05) reversed elevation of Na+ and Ca2+ concentration compared with sciatic nerve chronic constriction injury (snCCI) group (35.68 ± 1.71 vs 44.46 ± 1.24 mg/ml/mg protein and 1.06 ± 0.19 vs 6.66 ± 0.03 mg/ml/mg protein, respectively). There were also significant (p < 0.05) increases in the level of K+ (0.84 ± 0.02 vs 0.36 ± 0.05 mg/ml/mg protein) and Cl (18.51 ± 0.29 vs 15.82 ± 0.21 mg/ml/mg protein). Bromelain reduced the activities of Ca2+ electrogenic pumps significantly compared with snCCI. This study therefore suggests that bromelain mitigated electrolyte imbalance in chronic constricted injury of the sciatic nerve implying that this may be an important mechanism for the anti-nociceptive effect of bromelain.


Bromelain Chronic constriction injury Electrolyte imbalance Electrogenic pump Sciatic nerve 



Our special appreciation goes to Mr. Sodiq Ishola and Mr. Bakare Habeeb for their immense technical support during the course of this study.

Authors’ contributions

All the authors listed were involved in carrying out the laboratory works and contributed to the writing of the manuscripts.

Compliance with ethical standards

All experimental protocols were carried out in accordance with the International Ethical Guidelines (International Association for the Study of Pain). Ethical clearance was obtained from the University of Ilorin Ethical Review Committee (UERC/ASN/2017/936).

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Pain and Inflammation Unit, Department of PhysiologyUniversity of IlorinIlorinNigeria
  2. 2.Neuroscience Unit, Department of PhysiologyUniversity of IlorinIlorinNigeria

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