Casticin inhibits esophageal cancer cell proliferation and promotes apoptosis by regulating mitochondrial apoptotic and JNK signaling pathways
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Casticin, a flavonoid isolated from Vitex species, has been found to have anti-tumor property in multiple human cancers. The present study aimed to investigate the effect of casticin on the proliferation and apoptosis of esophageal cancer (EC) cells, and further illustrate the underlying mechanisms. In in vitro studies, human EC cell lines TE-1 and ECA-109 were treated with various concentrations of casticin (low-, middle-, and high-dose groups). The results showed that casticin dose-dependently inhibited the proliferation and clonogenicity of EC cells and induced cell cycle arrest in sub-G1 and G2 phases. Furthermore, casticin markedly enhanced EC cell apoptosis as detected by flow cytometry and Hoechst 33342 staining. The level of anti-apoptotic Bcl-2 protein was decreased, while the levels of pro-apoptotic Bax, cleaved-caspase-3, cleaved-caspase-9, and cleaved-PARP were conversely increased in casticin-treated TE-1 and ECA-109 cells. Moreover, casticin decreased the mitochondrial membrane potential and increased the release of mitochondrial cytochrome C into cytoplasm. In addition, the JNK signaling pathway was involved in casticin-medicated anti-proliferation and pro-apoptosis. Cells pretreated with SP600125, a JNK pathway inhibitor, partially abolished the effect of casticin. Finally, the anti-tumor property of casticin was confirmed in in vivo xenograft models. Overall, we provided both in vitro and in vivo evidences that casticin inhibited the proliferation and induced apoptosis of EC cells, and the anti-tumor action of casticin was mediated, in part, by the mitochondrial-dependent apoptosis and the activation of JNK signaling pathway.
KeywordsCasticin Esophageal cancer Mitochondrial apoptotic JNK Cell proliferation Apoptosis
ZQ conceived and designed study; ZQ, YC, and SLiu performed experiments; ZQ, ZM, SLi, and WZ analyzed data. ZQ and YC wrote the manuscript.
This study was supported by a grant from the Science and Technology Development Project of Shaanxi Province (No. S2015YFSF0111).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All the animal experiments were conducted in strict accordance with the Guidelines for the care and use of laborary animals and were approved by the Experimental Animal Ethics of the Second Affiliated Hospital of Xi’an Jiaotong University.
- Joza N, Susin SA, Daugas E, Stanford WL, Cho SK, Li CYJ, Sasaki T, Elia AJ, Cheng HYM, Ravagnan L, Ferri KF, Zamzami N, Wakeham A, Hakem R, Yoshida H, Kong YY, Mak TW, Zúñiga-Pflücker JC, Kroemer G, Penninger JM (2001) Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 410:549–554. https://doi.org/10.1038/35069004 CrossRefPubMedGoogle Scholar
- Lee H, Jung KH, Park S, Choi W, Bae H (2015) Casticin, an active compound isolated from Vitex Fructus, ameliorates the cigarette smoke-induced acute lung inflammatory response in a murine model. Int Immunopharmacol 28:1097–1101. https://doi.org/10.1016/j.intimp.2015.07.041 CrossRefPubMedGoogle Scholar
- Qu L, Liu FX, Cao XC, Xiao Q, Yang X, Ren KQ (2014) Activation of the apoptosis signal-regulating kinase 1/c-Jun N-terminal kinase pathway is involved in the casticin-induced apoptosis of colon cancer cells. Exp Ther Med 8:1494–1500. https://doi.org/10.3892/etm.2014.1934 CrossRefPubMedPubMedCentralGoogle Scholar
- Song XL, Zhang YJ, Wang XF, Zhang WJ, Wang Z, Zhang F, Zhang YJ, Lu JH, Mei JW, Hu YP, Chen L, Li HF, Ye YY, Liu YB, Gu J (2017) Casticin induces apoptosis and G0/G1 cell cycle arrest in gallbladder cancer cells. Cancer Cell Int 17:9. https://doi.org/10.1186/s12935-016-0377-3 CrossRefPubMedPubMedCentralGoogle Scholar