Predominant role of the dopamine D3 receptor subtype for mediating the quinpirole-induced inhibition of the vasopressor sympathetic outflow in pithed rats

  • Inna Ruiz-Salinas
  • Abimael González-Hernández
  • Guadalupe Manrique-Maldonado
  • Bruno A. Marichal-Cancino
  • Alain H. Altamirano-Espinoza
  • Carlos M. Villalón
Original Article
First Online:
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Accepted:

Abstract

We have recently reported that quinpirole (a D2-like receptor agonist) inhibits the vasopressor sympathetic outflow in pithed rats via sympatho-inhibitory D2-like receptors. Since D2-like receptors consist of D2, D3 and D4 receptor subtypes, this study investigated whether these subtypes are involved in the above quinpirole-induced sympatho-inhibition by using antagonists of these receptor subtypes. One hundred fifty-six male Wistar rats were pithed and prepared for preganglionic spinal (T7–T9) stimulation of the vasopressor sympathetic outflow. This approach resulted in frequency-dependent vasopressor responses which were analysed before and during i.v. continuous infusions of either saline (0.02 ml/min) or quinpirole (1 μg/kg.min) in animals receiving i.v. bolus injections of vehicle [saline or dimethyl sulfoxide (DMSO)] or the antagonists L-741,626 (D2), nafadotride or SB-277011-A (both D3) as well as L-745,870 (D4). Quinpirole inhibited the sympathetically-induced vasopressor responses. This sympatho-inhibition was (a) unaltered after 1 ml/kg saline, DMSO or 100 and 300 μg/kg L-741,626; (b) markedly blocked and abolished by, respectively, 30 and 100 μg/kg nafadotride or 100 and 300 μg/kg SB-277011-A and (c) slightly blocked after 30 and 100 μg/kg L-745,870, but 300 μg/kg L-745,870 produced no blockade whatsoever. Except for 300 μg/kg L-741,626 or 300 μg/kg L-745,870, the doses of the above compounds failed to modify per se the sympathetic vasopressor responses. The inhibition of the vasopressor sympathetic outflow induced by 1 μg/kg.min quinpirole in pithed rats is predominantly mediated by dopamine D3 and, to a lesser extent, by D4 receptor subtypes, with no evidence for the involvement of the D2 subtype.

Keywords

L-741,626 L-745,870 Nafadotride SB-277011-A Quinpirole Vasopressor sympathetic outflow 
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Notes

Acknowledgments

The authors would like to thank Mr. Mauricio Villasana for his skilful technical assistance. The present study was financially supported by the Consejo Nacional de Ciencia y Tecnología (CONACyT, project no. 60789; México DF).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Inna Ruiz-Salinas
    • 1
  • Abimael González-Hernández
    • 1
  • Guadalupe Manrique-Maldonado
    • 1
  • Bruno A. Marichal-Cancino
    • 1
  • Alain H. Altamirano-Espinoza
    • 1
  • Carlos M. Villalón
    • 1
  1. 1.Departamento de FarmacobiologíaCinvestav-CoapaMexico DFMexico

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