Inhibition of TNF-α protects against hepatic ischemia–reperfusion injury in rats via NF-κB dependent pathway

  • Mona F. Mahmoud
  • Shimaa M. El Shazly
  • Waleed Barakat
Original Article


Hepatic ischemia–reperfusion injury (I/R) is a serious health problem associated with liver transplantation, resection surgery, and various types of shock especially hemorrhagic shock. In the present investigation, the effect of inhibition of tumor necrosis factor-alpha (TNF-α) using pentoxifylline or infliximab against hepatic I/R injury induced in rats by 45-min ischemia and 1-h reperfusion was studied. It was observed that both pentoxifylline and infliximab-treated groups showed a significantly lower extent and severity of liver injury. This is attributed to (1) a decrease in oxidative stress markers, (2) reduction of the expression of TNF-α, TNF-α type-1 receptors, and nuclear factor kappa B (NF-κB). Thus TNF-α inhibition may be one of the therapeutic interventions to overcome the deleterious effects of I/R on liver via reduction of oxidative stress and inhibition of inflammatory cascade.


TNF-α Pentoxifylline Infliximab Ischemia/reperfusion injury 



The authors would like to thank Professor Laila Rashed (Biochemistry Department, Faculty of Medicine, Cairo University, Egypt) for assistance in performing PCR technique.

Conflicts of interest

The authors declare no conflict of interest.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Mona F. Mahmoud
    • 1
  • Shimaa M. El Shazly
    • 1
  • Waleed Barakat
    • 1
  1. 1.Department of Pharmacology and Toxicology, Faculty of PharmacyZagazig UniversityZagazigEgypt

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