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Antinociceptive and anti-inflammatory effects of a mucin-binding agglutinin isolated from the red marine alga Hypnea cervicornis

  • Flávio da S. Bitencourt
  • Jozi G. Figueiredo
  • Mário R. L. Mota
  • Carla C. R. Bezerra
  • Priscila P. Silvestre
  • Marcus R. Vale
  • Kyria S. Nascimento
  • Alexandre H. Sampaio
  • Celso S. Nagano
  • Silvana Saker-Sampaio
  • Wladimir R. L. Farias
  • Benildo S. Cavada
  • Ana M. S. AssreuyEmail author
  • Nylane M. N. de Alencar
Original Article

Abstract

The agglutinin from the red marine alga Hypnea cervicornis (HCA) was tested in models of nociception and inflammation. The role of carbohydrate-binding sites and the systemic toxicity were assessed. HCA (10−1, 1, and 10 mg/kg) administered i.v. to mice inhibited writhes induced by acetic acid and, at 10 mg/kg, inhibited the second phase of the formalin test, but did not alter the response latency in the hot-plate test. HCA (1 mg/kg) administered i.v. to rats reduced carrageenan-induced paw edema at 1, 2, and 3 h after challenge, but not edema induced by dextran. The neutrophil migration induced by both N-formyl-methionyl-leucyl-phenylalanine (fMLP) and carrageenan was inhibited by HCA at 10−1, 1, and 10 mg/kg. The combination of HCA (1 mg/kg) and its ligand mucin reversed the lectin inhibitory effect on carrageenan-induced neutrophil migration and acetic acid-induced writhes. The i.v. treatment of rats with HCA (1 mg/kg) for 7 days did not affect body mass; liver, kidney or heart wet weight; blood leukocyte counts; urea, creatinine or serum transaminase activity; or macroscopy of the organs examined. In short, H. cervicornis agglutinin showed important antinociceptive and anti-inflamatory activity via interaction with the lectin carbohydrate-binding site.

Keywords

Agglutinin Lectin Hypnea cervicornis Red marine alga Antinociceptive activity Anti-inflammatory activity 

Abbreviations

HCA

Hypnea cervicornis agglutinin

ALT

Alanine amine transferase

AST

Aspartate amine transferase

Cg

Carrageenan

fMLP

N-formyl-methionyl-leucyl-phenyl alanine

Dexa

Dexamethasone

Notes

Acknowledgements

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Cearense de Amparo à Pesquisa (FUNCAP). Drs. Alexandre H. Sampaio, Ana M. S. Assreuy, Benildo S. Cavada and Nylane M.N. de Alencar are senior investigators of CNPq (Brazil). Dr. A. Leyva provided English editing of the manuscript.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Flávio da S. Bitencourt
    • 1
  • Jozi G. Figueiredo
    • 2
  • Mário R. L. Mota
    • 1
  • Carla C. R. Bezerra
    • 1
  • Priscila P. Silvestre
    • 1
  • Marcus R. Vale
    • 1
  • Kyria S. Nascimento
    • 2
  • Alexandre H. Sampaio
    • 3
  • Celso S. Nagano
    • 3
  • Silvana Saker-Sampaio
    • 3
  • Wladimir R. L. Farias
    • 3
  • Benildo S. Cavada
    • 2
  • Ana M. S. Assreuy
    • 4
    Email author
  • Nylane M. N. de Alencar
    • 1
  1. 1.Department of Physiology and PharmacologyFederal University of CearáFortalezaBrazil
  2. 2.Department of Biochemistry and Molecular BiologyFederal University of CearáFortalezaBrazil
  3. 3.Department of Fishing EngineeringFederal University of CearáFortalezaBrazil
  4. 4.Superior Institute of Biomedical SciencesState University of CearáFortaleza-CearáBrazil

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