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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 368, Issue 5, pp 331–341 | Cite as

Different pattern of brain c-Fos expression following re-exposure to ethanol or sucrose self-administration environment

  • Krzysztof Wedzony
  • Eliza Koros
  • Anna Czyrak
  • Agnieszka Chocyk
  • Klaudia Czepiel
  • Katarzyna Fijal
  • Marzena Mackowiak
  • Artur Rogowski
  • Wojciech Kostowski
  • Przemyslaw BienkowskiEmail author
Original Article

Abstract

Exposure of alcohol addicts to alcohol-related environmental cues may elicit alcohol-seeking behavior and lead to relapse to heavy drinking. The aim of the present study was to identify brain regions activated by alcohol (ethanol)-related stimuli in Wistar rats trained to lever press for 8% ethanol solution in operant self-administration cages. Ethanol self-administration was stabilized in a maintenance phase, which lasted for 30 days. c-Fos protein expression was used as a marker of neuronal activation.

Re-exposure to ethanol self-administration environment after 30-day but not after 24-h abstinence increased the number of Fos-positive nuclei in the thalamic paraventricular nucleus, granular insular cortex and medial prefrontal cortex. In general, no differences were found in c-Fos protein expression between the rats allowed to self-administer alcohol and the subjects exposed only to alcohol-related stimuli. In contrast, no increase in c-Fos immunoreactivity was observed in rats trained to lever press for sucrose solution and exposed to sucrose-related environmental stimuli after 30-day abstinence.

Taken together, these results suggest that at least some thalamo-cortical circuits become more responsive to ethanol-paired stimuli after prolonged abstinence and that ethanol- and sucrose-seeking behavior may be regulated by partially different neural mechanism(s).

Keywords

Ethanol self-administration Extinction Reinstatement c-Fos Inducible transcription factors Brain mapping 

Notes

Acknowledgment

The study was supported by the State Committee for Scientific Research (grants no. 4P05A 08 317 and PBZ-KBN-033/P05/2002).

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Krzysztof Wedzony
    • 1
  • Eliza Koros
    • 2
  • Anna Czyrak
    • 1
  • Agnieszka Chocyk
    • 1
  • Klaudia Czepiel
    • 1
  • Katarzyna Fijal
    • 1
  • Marzena Mackowiak
    • 1
  • Artur Rogowski
    • 2
  • Wojciech Kostowski
    • 2
    • 3
  • Przemyslaw Bienkowski
    • 2
    Email author
  1. 1.Department of Pharmacology, Institute of PharmacologyPolish Academy of SciencesKrakowPoland
  2. 2.Department of PharmacologyInstitute of Psychiatry and NeurologyWarsawPoland
  3. 3.Department of Experimental and Clinical PharmacologyWarsaw Medical AcademyWarsawPoland

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