Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 366, Issue 6, pp 501–512

The uptake machinery of clostridial actin ADP-ribosylating toxins – a cell delivery system for fusion proteins and polypeptide drugs

  • Holger Barth
  • Dagmar Blöcker
  • Klaus Aktories
Review Article

Abstract.

Several bacterial protein toxins, including Clostridium botulinum C2 toxin, Clostridum perfringens iota toxin, Clostridium difficile ADP-ribosyltransferase, and the Bacillus-produced vegetative insecticidal proteins, target the cytoskeleton by ADP-ribosylation of actin. All these toxins are binary in structure and consist of an enzyme component, possessing ADP-ribosyltransferase activity and a separated binding and translocation component, which is involved in the delivery of the enzyme component into the cell. The toxins are not only important virulence factors but also cell biological tools to study the function of the actin cytoskeleton. Moreover, the binary toxins turned out to be effective transporter systems for the delivery of specific fusion toxins (e.g., Rho-ADP-ribosylating C3 exoenzyme) into cells. The present review describes the biological functions of the toxins, focuses on recent studies on the uptake and delivery mechanism and discusses the usage as a drug delivery system.

Clostridium botulinum C2 toxin Binary toxins Actin ADP-ribosylation Protein delivery system Fusion toxins 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Holger Barth
    • 1
  • Dagmar Blöcker
    • 1
  • Klaus Aktories
    • 1
  1. 1.Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Albert-Ludwigs-Universität Freiburg, Otto-Krayer-Haus, Albertstrasse 25, 79104 Freiburg; Germany

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