Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 365, Issue 4, pp 269–276 | Cite as

Antioxidative and myocardial protective effects of L-arginine in oxygen radical-induced injury of isolated perfused rat hearts

  • Astrid Suessenbacher
  • Achim Lass
  • Bernd Mayer
  • Friedrich Brunner
Original Article

Abstract.

Oxygen-derived free radicals and oxidants (reactive oxygen intermediates, ROI) have been implicated in cardiovascular diseases. The protective role of nitric oxide (NO) against ROI-mediated tissue injury is not resolved. We tested the effects of exogenous NO, L- and D-arginine and a NO synthase inhibitor on electrolysis-induced cardiac injury and the generation of ROI by electrolysis. Superoxide dismutase (SOD) and catalase were used for comparison.

Hearts (n=7) from male rats (350±30 g) were perfused in vitro at 10 ml min–1 g–1, ROI generated by electrolysis of the perfusion medium (15 mA, 10 s), and cardiac function and the level of isoluminol-derived chemiluminescence in electrolysed perfusion medium documented for 15 min (n=4). The ROI-induced maximal reduction of left ventricular developed pressure to 55±5% of baseline, and a 2.2±0.1-fold rise in coronary perfusion pressure 3 min after electrolysis, were prevented by SOD (50 U ml–1), catalase (100 U ml–1), S-nitroso-N-acetyl-D,L-penicillamine (SNAP, 100 nmol l–1); L-arginine (1 mmol l–1), NG-nitro-L-arginine (L-NNA, 200 µmol l–1) or D-arginine (1 mmol l–1). The effect of L-arginine was concentration dependent. In all cases, the beneficial effects were closely matched by a near-total reduction of ROI in the perfusion medium.

We conclude that, besides mimicking or enhancing NO activity, L-arginine and donor-derived exogenous NO are cardioprotective by reducing ROI-mediated tissue injury. The protective effect of L-NNA and D-arginine implies that the protection results from a direct chemical interaction between the drug and the oxidizing species.

Free radical scavenging L-arginine Superoxide dismutase Rat heart Contractile function Coronary circulation Cardioprotection Chemiluminescence 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Astrid Suessenbacher
    • 1
  • Achim Lass
    • 1
  • Bernd Mayer
    • 1
  • Friedrich Brunner
    • 1
  1. 1.Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, 8010 Graz, Austria

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