Archives of Toxicology

, Volume 72, Issue 10, pp 671–675 | Cite as

Quantitative analysis of O-isopropyl methylphosphonic acid in serum samples of Japanese citizens allegedly exposed to sarin: estimation of internal dosage

  • Daan Noort
  • Albert G. Hulst
  • Dominique H. J. M. Platenburg
  • Martine Polhuijs
  • Hendrik P. Benschop
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Abstract

A convenient and rapid micro-anion exchange liquid chromatography (LC) tandem electrospray mass spectrometry (MS) procedure was developed for quantitative analysis in serum of O-isopropyl methylphosphonic acid (IMPA), the hydrolysis product of the nerve agent sarin. The mass spectrometric procedure involves negative or positive ion electrospray ionization and multiple reaction monitoring (MRM) detection. The method could be successfully applied to the analysis of serum samples from victims of the Tokyo subway attack and of an earlier incident at Matsumoto, Japan. IMPA levels ranging from 2 to 135 ng/ml were found. High levels of IMPA appear to correlate with low levels of residual butyrylcholinesterase activity in the samples and vice versa. Based on our analyses, the internal and exposure doses of the victims were estimated. In several cases, the doses appeared to be substantially higher than the assumed lethal doses in man.

Key words Chemical warfare agents LC tandem MS Multiple reaction monitoring O-isopropyl methylphosphonic acid Sarin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Daan Noort
    • 1
  • Albert G. Hulst
    • 2
  • Dominique H. J. M. Platenburg
    • 1
  • Martine Polhuijs
    • 1
  • Hendrik P. Benschop
    • 1
  1. 1.Department of Chemical Toxicology, TNO Prins Maurits Laboratory, P.O. Box 45, NL-2280 AA Rijswijk, The NetherlandsNL
  2. 2.Department of Analysis of Toxic and Explosive Substances, TNO Prins Maurits Laboratory, P.O. Box 45, NL-2280 AA Rijswijk, The NetherlandsNL

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