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Archives of Toxicology

, Volume 71, Issue 11, pp 719–721 | Cite as

A note on the physiological background of the ethylene oxide adduct 7-(2-hydroxyethyl)guanine in DNA from human blood

  • Hermann M. Bolt
  • Monika Leutbecher
  • Klaus Golka
LETTER TO THE EDITOR

Abstract

A newly developed high-performance liquid chromatography (HPLC) method involves derivatization with phenylglyoxal and fluorescence detection, using 7-methylguanine as internal standard. The physiological background of the adduct 7-(2-hydroxyethyl)guanine in DNA isolated from human blood was determined by this method. In five persons the range of the adduct was between 2.1 and 5.8 pmol/mg DNA (mean 3.2). This finding is consistent with previous data obtained by others, using different analytical methods, and points to an intrinsic carcinogenic risk due to endogenous ethylene oxide.

Key words Ethylene Ethylene oxide 7-(2-Hydroxyethyl)guanine DNA adducts 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Hermann M. Bolt
    • 1
  • Monika Leutbecher
    • 1
  • Klaus Golka
    • 1
  1. 1.Institut für Arbeitsphysiologie an der Universität Dortmund, Ardeystrasse 67, D-44139 Dortmund, GermanyDE

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