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Archives of Toxicology

, Volume 70, Issue 10, pp 640–644 | Cite as

Inhibitory effect of atractylon on tert-butyl hydroperoxide induced DNA damage and hepatic toxicity in rat hepatocytes

  • Jin-Ming Hwang
  • Tsui-Hwa Tseng
  • Yih-Shou Hsieh
  • Feu-Pi Chou
  • Chau Jong Wang
  • C.-Y. Chu
ORIGINAL INVESTIGATION

Abstract

Atractylon, a main sesquiterpenic constituent of Atractylodes rhizomes, was studied for the mechanism of its inhibitory effects on the tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity and lipid peroxidation in primary culture of rat hepatocytes. In the preliminary study, atractylon showed an effective antioxidant property tested by its capacity for quenching 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Further investigations showed that atractylon at the concentrations of 0.01, 0.1 and 1.0 mg/ml decreased the formation of malondialdehyde (MDA), leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) and repair synthesis of DNA induced by 30-min treatment of t-BHP (1.5 mM) in primary cultured rat hepatocytes. Addition of atractylon also attenuated the genotoxicity of t-BHP evaluated by unscheduled DNA synthesis. The sum of the results suggested that the protective effect of atractylon against oxidative stress induced by t-BHP is via its ability to quench free radicals.

Key words Atractylon(Atr) tert-Butyl hydroperoxide (t-BHP) Lipid peroxidation Hepatotoxicity 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Jin-Ming Hwang
    • 1
  • Tsui-Hwa Tseng
    • 1
  • Yih-Shou Hsieh
    • 1
  • Feu-Pi Chou
    • 1
  • Chau Jong Wang
    • 1
  • C.-Y. Chu
    • 1
  1. 1.Institute of Biochemistry, Chung Shan Medical and Dental College, No. 113, Section 2, Ta-Ching Street, Taichung 402, Taiwan, Republic of ChinaTW

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