Archives of Toxicology

, Volume 75, Issue 4, pp 209–213

Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter

  •  Y. Maruyama
  •  Y. Suzuki
  •  A. Kazusaka
  •  S. Fujita
Molecular Technology

DOI: 10.1007/s002040000202

Cite this article as:
Maruyama, Y., Suzuki, Y., Kazusaka, A. et al. Arch Toxicol (2001) 75: 209. doi:10.1007/s002040000202

Abstract.

The uptake of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, was studied in PC12 cells. The compound was actively taken up by the PC12 cells, with a Km value of 176.2±9.1 µM and a maximum velocity of 55.6±7.0 pmol/min per mg protein; norsalsolinol uptake was dependent on the presence of extracellular Na+. The uptake of norsalsolinol was sensitive to two dopamine transporter inhibitors, GBR-12909 and reserpine, but was less sensitive to desipramine, a noradrenaline transporter inhibitor. Dopamine competitively inhibited norsalsolinol uptake into PC12 cells with a Ki value of 271.2±61.6 µM. These results suggest that norsalsolinol is taken up into PC12 cells mainly by the dopamine transporter.

Dopamine transporter Norsalsolinol Parkinsonism PC12 cells 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  Y. Maruyama
    • 1
  •  Y. Suzuki
    • 1
  •  A. Kazusaka
    • 1
  •  S. Fujita
    • 1
  1. 1.Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan

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