Re: Gi et al. 2018, In vivo positive mutagenicity of 1,4-dioxane and quantitative analysis of its mutagenicity and carcinogenicity in rats, Archives of Toxicology 92:3207–3221
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Gi et al. recently published an in vivo genotoxicity study of 1,4-dioxane, examining, amongst other endpoints, treatment-induced transgene mutations in the livers of gpt delta rats (Gi et al. 2018). The authors employed the BMDS and PROAST software packages to analyze the dose–response data and determine a point of departure (PoD) metric known as the BMD or Benchmark Dose. With respect to BMDS, the authors used one standard deviation of the concurrent control group as the benchmark response (BMR), and determined the lower confidence limit of the BMD1SD (i.e., the BMDL1SD). With respect to PROAST, they used 10% increase over the mean concurrent control group as the BMR, also known as the critical effect size (CES), and determined the lower confidence limit of the BMD10 (i.e., the BMDL10). The authors also examined the no observed effect level (NOEL), the highest tested dose that failed to elicit a significant increase in response relative to the concurrent control.
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