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Archives of Toxicology

, Volume 92, Issue 2, pp 571–586 | Cite as

Comparative developmental toxicity of a comprehensive suite of polycyclic aromatic hydrocarbons

  • Mitra C. Geier
  • Anna C. Chlebowski
  • Lisa Truong
  • Staci L. Massey Simonich
  • Kim A. Anderson
  • Robert L. Tanguay
Regulatory Toxicology

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental contaminants that occur in complex mixtures. Several PAHs are known or suspected mutagens and/or carcinogens, but developmental toxicity data is lacking for PAHs, particularly their oxygenated and nitrated derivatives. Such data are necessary to understand and predict the toxicity of environmental mixtures. 123 PAHs were assessed for morphological and neurobehavioral effects for a range of concentrations between 0.1 and 50 µM, using a high throughput early-life stage zebrafish assay, including 33 parent, 22 nitrated, 17 oxygenated, 19 hydroxylated, 14 methylated, 16 heterocyclic, and 2 aminated PAHs. Additionally, each PAH was evaluated for AHR activation, by assessing CYP1A protein expression using whole animal immunohistochemistry (IHC). Responses to PAHs varied in a structurally dependent manner. High-molecular weight PAHs were significantly more developmentally toxic than the low-molecular weight PAHs, and CYP1A expression was detected in five distinct tissues, including vasculature, liver, skin, neuromasts and yolk.

Keywords

Zebrafish PAH CYP1A Developmental toxicity AHR 

Supplementary material

204_2017_2068_MOESM1_ESM.pdf (566 kb)
Supplementary material 1 (PDF 566 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of Environmental and Molecular ToxicologyOregon State UniversityCorvallisUSA

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