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Archives of Toxicology

, Volume 89, Issue 8, pp 1337–1346 | Cite as

Toxicological interactions between the mycotoxins deoxynivalenol, nivalenol and their acetylated derivatives in intestinal epithelial cells

  • Imourana Alassane-Kpembi
  • Olivier Puel
  • Isabelle P. OswaldEmail author
In vitro systems

Abstract

In case of mycotoxin contaminations, food and feedstuff are usually contaminated by more than one toxin. However toxicological data concerning the effects of mycotoxin combinations are sparse. The intestinal epithelium is the first barrier against food contaminants and this constantly renewing organ is particularly sensitive to mycotoxins. The aim of this study was to investigate the effects of deoxynivalenol (DON) and four other type B trichothecenes (TCTB), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), nivalenol (NIV) and fusarenon-X (FX) alone or in combination on intestinal epithelial cells. Proliferating, non-transformed IPEC-1 cells were exposed to increasing doses of TCTB, alone or in binary mixtures and mycotoxin-induced cytotoxicity was measured with MTT test. The toxicological interactions were assessed using the isobologram-Combination index method. The five tested mycotoxins and their mixtures had a dose-dependent effect on the proliferating enterocytes. DON–NIV, DON–15-ADON and 15-ADON–3-ADON combinations were synergistic, with magnitude of synergy for 10 % cytotoxicity ranging from 2 to 7. The association between DON and 3-ADON also demonstrated a synergy but only at high doses, at lower doses antagonism was noted. Additivity was observed between NIV and FX, and antagonism between DON and FX. These results indicate that the simultaneous presence of mycotoxins in food commodities and diet may be more toxic than predicted from the mycotoxins alone. This synergy should be taken into account considering the frequent co-occurrence of TCTB in the diet.

Keywords

Combination index Cytotoxicity Non-transformed intestinal cell Mycotoxin Synergy Trichothecenes 

Abbreviations

3-ADON

3-Acetyldeoxynivalenol

15-ADON

15-Acetyldeoxynivalenol

CI

Combination index

DON

Deoxynivalenol

Dm

Median effect dose

DRI

Dose reduction index

fa

Fraction affected

FX

Fusarenon-X

IC50

Inhibitory concentration 50 %

MTT

3,(4,5-Dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide

NIV

Nivalenol

Notes

Acknowledgments

The authors are grateful to Pr. T.C. Chou, Memorial Sloan-Kettering Cancer Center, New York City for kind help in data analysis, and Pr F. A. Abiola, Institut des Sciences Biomédicales Appliquées, Cotonou (Republic of Benin) for fruitful discussion. The authors thank Dr. Woodley for language editing. This work was supported by the ANR-CESA project DON & Co. I.A.K. was supported by a doctoral fellowship from the Government of the Republic of Benin.

Conflict of interest

Authors declare no conflict of interest.

Supplementary material

204_2014_1309_MOESM1_ESM.pptx (289 kb)
Supplementary material 1 (PPTX 289 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Imourana Alassane-Kpembi
    • 1
    • 2
    • 3
  • Olivier Puel
    • 1
    • 2
  • Isabelle P. Oswald
    • 1
    • 2
    Email author
  1. 1.UMR 1331 Toxalim, Research Center in Food ToxicologyINRAToulouse cedex 03France
  2. 2.INP, UMR 1331, ToxalimUniversité de ToulouseToulouseFrance
  3. 3.Hôpital d’Instruction des ArméesCotonouBénin

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