Archives of Toxicology

, Volume 88, Issue 9, pp 1739–1748 | Cite as

TCDD-induced chick cardiotoxicity is abolished by a selective cyclooxygenase-2 (COX-2) inhibitor NS398

  • Nozomi Fujisawa
  • Shouta M. M. Nakayama
  • Yoshinori Ikenaka
  • Mayumi Ishizuka
Organ Toxicity and Mechanisms


Halogenated aromatic hydrocarbons, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), are known to cause severe heart defects in avian species. However, the mechanism of TCDD-induced chick cardiovascular toxicity is unclear. In this study, we investigated cyclooxygenase-2 (COX-2) as a possible mechanism of TCDD-induced cardiotoxicity. Fertile chicken eggs were injected with TCDD and a COX-2 selective inhibitor, NS398, and we investigated chick heart failure on day 10. We found that the chick heart to body weight ratio and atrial natriuretic factor mRNA expression were increased, but this increase was abolished with treatment of NS398. In addition, the morphological abnormality of an enlarged ventricle resulting from TCDD exposure was also abolished with co-treatment of TCDD and NS398. Our results suggested that TCDD-induced chick heart defects are mediated via the nongenomic pathway and that they do not require the genomic pathway.


Atrial natriuretic factor Cardiac malformation Chick embryo Cyclooxygenase-2 inhibitor Nongenomic pathway TCDD 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nozomi Fujisawa
    • 1
  • Shouta M. M. Nakayama
    • 1
  • Yoshinori Ikenaka
    • 1
  • Mayumi Ishizuka
    • 1
  1. 1.Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan

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