Archives of Toxicology

, Volume 87, Issue 12, pp 2151–2163 | Cite as

Rapeseed oil-rich diet alters hepatic mitochondrial membrane lipid composition and disrupts bioenergetics

  • João P. Monteiro
  • Cláudia V. Pereira
  • Ana M. Silva
  • Elisabete Maciel
  • Inês Baldeiras
  • Francisco Peixoto
  • Maria R. Domingues
  • Amália S. Jurado
  • Paulo J. Oliveira
Molecular Toxicology

Abstract

Diet is directly related with physiological alterations occurring at a cell and subcellular level. However, the role of diet manipulation on mitochondrial physiology is still largely unexplored. Aiming at correlating diet with alterations of mitochondrial membrane composition and bioenergetics, Wistar-Han male rats were fed for 11, 22 and 33 days with a rapeseed oil-based diet and mitochondrial bioenergetics, and membrane composition were compared at each time point with a standard diet group. Considerable differences were noticed in mitochondrial membrane lipid composition, namely in terms of fatty acyl chains and relative proportions of phospholipid classes, the modified diet inducing a decrease in the saturated to unsaturated molar ratio and an increase in the phosphatidylcholine to phosphatidylethanolamine molar ratio. Mass spectrometry lipid analysis showed significant differences in the major species of cardiolipin, with an apparent increased incorporation of oleic acid as a result of exposure to the modified diet. Rats fed the modified diet during 22 days showed decreased hepatic mitochondrial state 3 respiration and were more susceptible to Ca2+-induced transition pore opening. Rapeseed oil-enriched diet also appeared to promote a decrease in hydroperoxide production by the respiratory chain, although a simultaneous decrease in vitamin E content was detected. In conclusion, our data indicate that the rapeseed oil diet causes negative alterations on hepatic mitochondrial bioenergetics, which may result from membrane remodeling. Such alterations may have an impact not only on energy supply to the cell, but also on drug-induced hepatic mitochondrial liabilities.

Keywords

Rapeseed oil Diet Liver mitochondria Wistar rat Toxicity Mitochondrial membrane 

Notes

Acknowledgments

The project was supported by the Foundation for Science and Technology with FEDER/COMPETE/National Budget funds (research grants PTDC/QUI–QUI/101409/2008 to P. J. O., PTDC/QUI-BIQ/103001/2008 to A. S. J. and strategic grant PEst-C/SAU/LA0001/2011to the CNC). J. P. M. and A. M. S. acknowledge FCT for Ph.D. grants SFRH/BD/37626/2007 and PTDC/AGR-ALI/108326/2008, respectively.

Conflict of interest

The authors declare that they have no conflicts of interest. The Funding Agency, which is supported by the Portuguese Government, had no role in the decision to publish the data or in the data presented.

Supplementary material

204_2013_1068_MOESM1_ESM.pdf (379 kb)
Supplementary material 1 (PDF 379 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • João P. Monteiro
    • 1
    • 2
  • Cláudia V. Pereira
    • 1
  • Ana M. Silva
    • 1
  • Elisabete Maciel
    • 3
  • Inês Baldeiras
    • 1
    • 4
  • Francisco Peixoto
    • 5
  • Maria R. Domingues
    • 3
  • Amália S. Jurado
    • 1
    • 2
  • Paulo J. Oliveira
    • 1
  1. 1.CNC—Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbraPortugal
  2. 2.Department of Life SciencesUniversity of CoimbraCoimbraPortugal
  3. 3.Department of Chemistry, Mass Spectrometry Centre, QOPNAUniversity of AveiroAveiroPortugal
  4. 4.Neurological Clinic, Faculty of MedicineUniversity of CoimbraCoimbraPortugal
  5. 5.CECAV—Centre of Animal Sciences and Veterinary/Chemistry DepartmentUniversity of Trás-os-Montes and Alto DouroVila RealPortugal

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