Archives of Toxicology

, Volume 87, Issue 7, pp 1219–1230 | Cite as

Comparison of toxicity between the different-type TiO2 nanowires in vivo and in vitro

  • Eun-Jung Park
  • Hyun-Woo Shim
  • Gwang-Hee Lee
  • Jae-Ho Kim
  • Dong-Wan Kim
Inorganic Compounds


In this study, we compared their toxicity in vivo and in vitro based on the physicochemical properties of three different types of TiO2 nanowires, H2Ti3O7 nanowires (1HTO), hydrothermal treatment (2HTO), and calcination (3HTO) of 1HTO. The surface of 1HTO was smooth, and the surface of 2HTO was much rougher. The negative charge on the surface increased in the order of 2HTO, 3HTO, and 1HTO, whereas the surface area increased in the order of 3HTO, 1HTO, and 2HTO. The lung is a main exposure route of nanoparticles. On day 28 after a single instillation (1 mg/kg), three nanowires induced a Th2-type inflammatory response together with the relative increase in CD4+ T cells, especially by 2HTO. In vitro, three TiO2 nanowires (10 μg/ml) commonly induced the generation of cell debris in eight cell lines which may be the potential target organ of nanoparticles, especially by 2HTO. It seemed that the generation of cell debris coincides with the increase in autophagosome-like vacuoles in the cytosol. In further study using BEAS-2B cells originated from the lung, the protein amount from cells exposed to 2HTO decreased more clearly although the generation of reactive oxygen species (ROS) was less compared to 1HTO and 3HTO. Based on these results, we suggest that surface area may act as an important factor depends on the biological response by TiO2 nanowires. Furthermore, the increase in autophagosome-like vacuoles may be an important cause of cell death by nanoparticles with ROS.


Titanium Nanowires Toxicity Autophagosome Surface area Vacuoles 



This work was supported by the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2011-35B-E00011, NRF-2011-0030745).

Supplementary material

204_2013_1019_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)
204_2013_1019_MOESM2_ESM.pdf (1.5 mb)
Supplementary material 2 (PDF 1496 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Eun-Jung Park
    • 1
  • Hyun-Woo Shim
    • 2
  • Gwang-Hee Lee
    • 2
  • Jae-Ho Kim
    • 1
  • Dong-Wan Kim
    • 2
  1. 1.Department of Molecular Science and TechnologyAjou UniversitySuwonKorea
  2. 2.Department of Materials Science and EngineeringAjou UniversitySuwonKorea

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