Archives of Toxicology

, Volume 86, Issue 11, pp 1795–1797 | Cite as

Reply to comment of John M. DeSesso and Amy L. Williams regarding “Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression” by Romano et al. 2012

  • Marco A. Romano
  • Renata M. Romano

The product used in our study was a commercial formulation of glyphosate Roundup Transorb (Monsanto, Brazil). In previous studies, we and other colleagues used different commercial formulations and observed a reduction in testosterone concentrations or reduction in StAR protein expression (Benachour et al. 2007; Benachour and Seralini 2009; Dallegrave et al. 2007; Gasnier et al. 2009; Richard et al. 2005; Romano et al. 2010; Walsh et al. 2000). The reduction in serum testosterone concentration was also observed in drakes exposed to a commercial formulation of glyphosate (Oliveira et al. 2007). Recently, it was observed that both glyphosate salt and a commercial formulation were able to reduce the testosterone production in 35 % (Clair et al. 2012).

Based on this, we proposed to investigate the effect of the reduction in the testosterone during the fetal and postnatal development, which is recognized as the hypothalamic sexual differentiation phase. The dams were exposed to the product...


Testosterone Glyphosate Male Offspring Commercial Formulation Material Safety Data Sheet 
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  1. Andrews P, Freyberger A, Hartmann E, Eiben R, Loof I, Schmidt U, Temerowski M, Becka M (2001) Feasibility and potential gains of enhancing the subacute rat study protocol (OECD test guideline no. 407) by additional parameters selected to determine endocrine modulation. A pre-validation study to determine endocrine-mediated effects of the antiandrogenic drug flutamide. Arch Toxicol 75:65–73. doi: 10.1007/s002040100214 PubMedCrossRefGoogle Scholar
  2. Benachour N, Seralini GE (2009) Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chem Res Toxicol 22:97–105. doi: 10.1021/tx800218n PubMedCrossRefGoogle Scholar
  3. Benachour N, Sipahutar H, Moslemi S, Gasnier C, Travert C, Seralini GE (2007) Time and dose-dependent effects of roundup on human embryonic and placental cells. Arch Environ Contam Toxicol 53:126–133. doi: 10.1007/s00244-006-0154-8 PubMedCrossRefGoogle Scholar
  4. Clair E, Mesnage R, Travert C, Séralini GÉ (2012) A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro, and testosterone decrease at lower levels. Toxicol In Vitro 26(2):269–279. doi: org/10.1016/j.tiv.2011.12.009 PubMedCrossRefGoogle Scholar
  5. Dallegrave E, Mantese FD, Oliveira RT, Andrade AJ, Dalsenter PR, Langeloh A (2007) Pre- and postnatal toxicity of the commercial glyphosate formulation in Wistar rats. Arch Toxicol 81(9):665–673. doi: 10.1007/s00204-006-0170-5 PubMedCrossRefGoogle Scholar
  6. DeSesso JM, Williams AL (2012) Comment on “Glyphosphate impairs male offspring reproductive development by disrupting gonadotropin expression” by Romano et al. (2012). Arch Toxicol 86 (in press)Google Scholar
  7. Gasnier C, Dumont C, Nora Benachour N, Clair E, Chagnon MC, Seralini GE (2009) Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines. Toxicology 262:184–191. doi: org/10.1016/j.tox.2009.06.006 PubMedCrossRefGoogle Scholar
  8. Ghanadian R, Lewis JG, Chisholm GD (1975) Serum testosterone and dihydrotestosterone changes with age in rat. Steroids 25:753–762. doi: org/10.1016/0039-128X(75)90039-2 PubMedCrossRefGoogle Scholar
  9. Haavisto T, Nurmela K, Pohjanvirta R, Huuskonen H, El-Gehani F, Paranko J (2001) Prenatal testosterone and luteinizing hormone levels in male rats exposed during pregnancy to 2,3,7,8-tetrachlorodibenzo-p-dioxin and diethylstilbestrol. Mol Cell Endocrinol 178:169–179. doi: org/10.1016/S0303-7207(01)00425-7 PubMedCrossRefGoogle Scholar
  10. Kennedy GC, Mitra J (1963) Body weight and food intake as initiating factors for puberty in the rat. J Physiol 166:408–418Google Scholar
  11. Korenbrot CC, Huhtaniemi IT, Weiner RI (1997) Preputial separation as an external sign of pubertal development in the male rat. Biol Reprod 17:298–303. doi: 10.1095/biolreprod17.2.298 CrossRefGoogle Scholar
  12. Mathias FT, Romano RM, Sleiman HK, Oliveira CA, Romano MA (2012) Herbicide metolachlor causes changes in reproductive endocrinology of male wistar Rats. ISRN Toxicology, Article ID 130846, p 7 doi: 10.5402/2012/130846
  13. Oliveira AG, Telles LF, Hess RA, Mahecha GA, Oliveira CA (2007) Effects of the herbicide Roundup on the epididymal region of drakes Anas platyrhynchos. Reprod Toxicol 23(2):182–191. doi: org/10.1016/j.reprotox.2006.11.004 PubMedCrossRefGoogle Scholar
  14. Parker RM (2006) Testing for reproductive toxicity. In: Hood RD (ed) Developmental and reproductive toxicology. Taylor and Francis, New York, pp 425–487Google Scholar
  15. Richard S, Moslemi S, Sipahutar H, Benachour N, Seralini G (2005) Differential effects of glyphosate and roundup on human placental cells. Environ Health Perspect 113:716–720. doi: 10.1289/ehp.7728 PubMedCrossRefGoogle Scholar
  16. Robaire B, Ewing LL, Irby DC, Desjardins C (1979) Interactions of testosterone and Estradiol-17β on the reproductive tract of the male rat. Biol Reprod 21:455–463. doi: 10.1095/biolreprod21.2.455 PubMedCrossRefGoogle Scholar
  17. Romano RM, Romano MA, Bernardi MM, Furtado PV, Oliveira CA (2010) Prepubertal exposure to commercial formulation of the herbicide Glyphosate alters testosterone levels and testicular morphology. Arch Toxicol 84(4):309–317. doi: 10.1007/s00204-009-0494-z PubMedCrossRefGoogle Scholar
  18. Romano MA, Romano RM, Santos LD, Wisniewski P, Campos DA, de Souza PB, Viau P, Bernardi MM, Nunes MT, Oliveira CA (2012a) Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression. Arch Toxicol 86(4):663–673. doi: 10.1007/s00204-011-0788-9 PubMedCrossRefGoogle Scholar
  19. Romano RM, Souza PB, Nunes MT, Romano MA (2012b) Perinatal exposure to a commercial formulation of glyphosate reduces the mRNA expression and increases the protein content of beta TSH in the pituitary of male offspring. Endocr Abstr 29:P75Google Scholar
  20. Vogel JM (2005) Perils of paradigm: complexity, policy design, and the endocrine disruptor screening program. Environ Health Glob Access Sci Source 4:2. doi: 10.1186/1476-069X-4-2 Google Scholar
  21. Walsh LP, McCormick C, Martin C, Stocco DM (2000) Roundup inhibits steroidogenesis by disrupting steroidogenic acute regulatory (StAR) protein expression. Environ Health Perspect 108:769–776PubMedCrossRefGoogle Scholar
  22. Ward GR, Abdel-Rahman AA (2005) Effect of testosterone replacement or duration of castration on baroreflex bradycardia in conscious rats. BMC Pharmacol 5:9. doi: 10.1186/1471-2210-5-9 PubMedCrossRefGoogle Scholar
  23. Zabka AG, Mitchell GS, Behansion M (2006) Conversion from testosterone to oestradiol is required to modulate respiratory long-term facilitation in male rats. J Physiol 576:903–912. doi: 10.1113/jphysiol.2006.114850 PubMedCrossRefGoogle Scholar
  24. Zoeller RT, Brown TR, Doan LL, Gore AC, Skakkebaek NE, Soto AM, Woodruff TJ, Vom Saal FS (2012) Endocrine-disrupting chemicals and public health protection: a statement of principles from The Endocrine Society. Endocrinology, [In press] doi: 10.1210/en.2012-1422

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Departamento de FarmaciaUniversidade Estadual do Centro-OesteGuarapuavaBrazil
  2. 2.Setor de Endocrinologia Experimental, Departamento de FarmacologiaUniversidade Federal de São PauloSão PauloBrazil
  3. 3.Departamento de Reproducao Animal, Faculdade de Medicina Veterinaria e ZootecniaUniversidade Federal de São PauloSão PauloBrazil

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