Waterpipe smoking: the role of humectants in the release of toxic carbonyls
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In recent years, the number of waterpipe smokers has increased substantially worldwide. Here, we present a study on the identification and quantification of seven carbonylic compounds including formaldehyde, acetaldehyde and acrolein in the mainstream smoke of the waterpipe. Smoking was conducted with a smoking machine, and carbonyls were scavenged from the smoke with two impingers containing an acidic solution of 2,4-dinitrophenylhydrazine. The derivatives were then analyzed by high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS). For instance, during one waterpipe smoking session, up to 111 ± 12 μg formaldehyde could be detected. This value is about 5 times higher when compared to one 2R4F reference cigarette. We also found a distinct filter effect of the bowl water for all carbonyls investigated. Our data further demonstrate that increasing amounts of humectants in the unburned tobacco lowers the temperature in the waterpipe head during smoking, thereby resulting in decreasing levels of carbonyls in the smoke produced. Altogether, considerable amounts of toxic carbonyls are present in the waterpipe smoke, thus conferring a health risk to waterpipe smokers.
KeywordsWaterpipe Carbonyls Formaldehyde Humectants LC–MS/MS
This work was supported by an internal grant of the German Federal Institute for Risk Assessment (BfR).
Conflict of interest
The authors declare that they have no conflict of interest.
- German Industrial Norm (DIN) (2008) Chemical analysis—decision limit, detection limit and determination limit under repeatability conditions—terms, methods, evaluation. Beuth Publishing Berlin DIN 32645:2008–2011Google Scholar
- German Tobacco Regulation (2010) Regulation on tobacco products (TabV 1977) as amended on 2010 (available in German only). http://www.gesetze-im-internet.de/bundesrecht/tabv_1977/gesamt.pdf. Accessed 07 Feb 2012
- Health Canada—Official Method (1999a) Determination of selected carbonyls in mainstream Tobacco Smoke vol 104. Ottawa, pp 1–13Google Scholar
- Health Canada—Official Method (1999b) Determination of humectants in whole tobacco vol 304. Ottawa, pp 1–7Google Scholar
- IARC (International Agency for Research on Cancer) (1999) IARC monographs on the evaluations of carcinogenicity to humans. Re-evaluation of some organic chemicals, hydrazine and hydrogen peroxide. IARC, LyonGoogle Scholar
- IARC (International Agency for Research on Cancer) (2006) IARC monographs on the evaluations of carcinogenicity to humans. Formaldehyde, 2-butoxyethanol and 1-tert-butoxypropan-2-ol. IARC, LyonGoogle Scholar
- Intorp M, Purkis S, Whittaker M, Wright W (2009) Determination of “Hoffmann analytes” in cigarette mainstream smoke. The Coresta 2006 joint experiment. Contrib Tob Res 23:161–202Google Scholar
- Moldoveanu S, Coleman W III, Wilkins J (2007) Determination of carbonyl compounds in exhaled cigarette smoke. Contrib Tob Res 22:346–357Google Scholar
- Yip SH, Taylor LT, Ashraf-Khorassani M, Yu J, Borgerding MF, Coleman WM III, Bodnar JA (2010) HPLC–MS determination of acrolein and acetone generated from 13C3-labeled glycerol added to cigarette tobacco using two machine-smoking regimes. Contrib Tob Res 24:48–57Google Scholar