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Archives of Toxicology

, Volume 86, Issue 8, pp 1309–1316 | Cite as

Waterpipe smoking: the role of humectants in the release of toxic carbonyls

  • Jens Schubert
  • Volkmar Heinke
  • Jana Bewersdorff
  • Andreas Luch
  • Thomas G. Schulz
Genotoxicity and Carcinogenicity

Abstract

In recent years, the number of waterpipe smokers has increased substantially worldwide. Here, we present a study on the identification and quantification of seven carbonylic compounds including formaldehyde, acetaldehyde and acrolein in the mainstream smoke of the waterpipe. Smoking was conducted with a smoking machine, and carbonyls were scavenged from the smoke with two impingers containing an acidic solution of 2,4-dinitrophenylhydrazine. The derivatives were then analyzed by high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS). For instance, during one waterpipe smoking session, up to 111 ± 12 μg formaldehyde could be detected. This value is about 5 times higher when compared to one 2R4F reference cigarette. We also found a distinct filter effect of the bowl water for all carbonyls investigated. Our data further demonstrate that increasing amounts of humectants in the unburned tobacco lowers the temperature in the waterpipe head during smoking, thereby resulting in decreasing levels of carbonyls in the smoke produced. Altogether, considerable amounts of toxic carbonyls are present in the waterpipe smoke, thus conferring a health risk to waterpipe smokers.

Keywords

Waterpipe Carbonyls Formaldehyde Humectants LC–MS/MS 

Notes

Acknowledgments

This work was supported by an internal grant of the German Federal Institute for Risk Assessment (BfR).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

204_2012_884_MOESM1_ESM.doc (806 kb)
Supplementary material 1 (DOC 805 kb)

References

  1. Al Rashidi M, Shihadeh A, Saliba NA (2008) Volatile aldehydes in the mainstream smoke of the narghile waterpipe. Food Chem Toxicol 46:3546–3549PubMedCrossRefGoogle Scholar
  2. Azab M, Khabour OF, Alkaraki AK, Eissenberg T, Alzoubi KH, Primack BA (2010) Water pipe tobacco smoking among university students in Jordan. Nicotine Tob Res 12:606–612PubMedCrossRefGoogle Scholar
  3. Beauchamp RO, Andielkovich DA, Kligerman AD, Morgan KT, Heck HD (1985) A critical review of the literature on acrolein toxicity. CRC Crit Rev Toxicol 14:309–380CrossRefGoogle Scholar
  4. Chi Y, Feng Y, Wen S, Lü H, Yu Z, Zhang W, Sheng G, Fu J (2007) Determination of carbonyl compounds in the atmosphere by DNPH derivatization and LC–ESI–MS/MS detection. Talanta 72:539–545PubMedCrossRefGoogle Scholar
  5. Cordis GA, Das DK, Riedel W (1998) High-performance liquid chromatographic peak identification of 2,4-dinitrophenylhydrazine derivatives of lipid peroxidation aldehydes by photodiode array detection. J Chromatogr A 798:117–123PubMedCrossRefGoogle Scholar
  6. Dong J, Moldoveanu SC (2004) Gas chromatography-mass spectrometry of carbonyl compounds in cigarette mainstream smoke after derivatization with 2,4-dinitrophenylhydrazine. J Chromatogr A 1027:25–35PubMedCrossRefGoogle Scholar
  7. Feng Z, Hu W, Hu Y, Tang MS (2006) Acrolein is a major cigarette-related lung cancer agent: preferential binding at p53 mutational hotspots and inhibition of DNA repair. Proc Natl Acad Sci USA 103:15404–15409PubMedCrossRefGoogle Scholar
  8. Fowles J, Dybing E (2003) Application of toxicological risk assessment principles to the chemical constituents of cigarette smoke. Tob Control 12:424–430PubMedCrossRefGoogle Scholar
  9. Gabrio T, Bertsch A (2004) Determination of carbonyl compounds in pool water with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride and gas chromatographic-tandem mass spectrometric analysis. J Chromatogr A 1046:293–296PubMedGoogle Scholar
  10. German Industrial Norm (DIN) (2008) Chemical analysis—decision limit, detection limit and determination limit under repeatability conditions—terms, methods, evaluation. Beuth Publishing Berlin DIN 32645:2008–2011Google Scholar
  11. German Tobacco Regulation (2010) Regulation on tobacco products (TabV 1977) as amended on 2010 (available in German only). http://www.gesetze-im-internet.de/bundesrecht/tabv_1977/gesamt.pdf. Accessed 07 Feb 2012
  12. Hammal F, Mock J, Ward KD, Eissenberg T, Maziak W (2008) A pleasure among friends: how narghile (waterpipe) smoking differs from cigarette smoking in Syria. Tob Control 17:e3PubMedCrossRefGoogle Scholar
  13. Health Canada—Official Method (1999a) Determination of selected carbonyls in mainstream Tobacco Smoke vol 104. Ottawa, pp 1–13Google Scholar
  14. Health Canada—Official Method (1999b) Determination of humectants in whole tobacco vol 304. Ottawa, pp 1–7Google Scholar
  15. Hoffmann D, Hoffmann I, El-Bayoumy K (2001) The less harmful cigarette: a controversial issue. A tribute to Ernst L. Wynder. Chem Res Toxicol 14:767–790PubMedCrossRefGoogle Scholar
  16. IARC (International Agency for Research on Cancer) (1999) IARC monographs on the evaluations of carcinogenicity to humans. Re-evaluation of some organic chemicals, hydrazine and hydrogen peroxide. IARC, LyonGoogle Scholar
  17. IARC (International Agency for Research on Cancer) (2006) IARC monographs on the evaluations of carcinogenicity to humans. Formaldehyde, 2-butoxyethanol and 1-tert-butoxypropan-2-ol. IARC, LyonGoogle Scholar
  18. Intorp M, Purkis S, Whittaker M, Wright W (2009) Determination of “Hoffmann analytes” in cigarette mainstream smoke. The Coresta 2006 joint experiment. Contrib Tob Res 23:161–202Google Scholar
  19. Jackson D, Aveyard P (2008) Waterpipe smoking in students: prevalence, risk factors, symptoms of addiction, and smoke intake. Evidence from one British University. BMC Public Health 8:174PubMedCrossRefGoogle Scholar
  20. Maziak W (2011) The global epidemic of waterpipe smoking. Addict Behav 36:1–5PubMedCrossRefGoogle Scholar
  21. Moldoveanu S, Coleman W III, Wilkins J (2007) Determination of carbonyl compounds in exhaled cigarette smoke. Contrib Tob Res 22:346–357Google Scholar
  22. Nimlos MR, Blanksby SJ, Qian X, Himmel ME, Johnson DK (2006) Mechanisms of glycerol dehydration. J Phys Chem A 110:6145–6156PubMedCrossRefGoogle Scholar
  23. Rakower J, Fatal B (1962) Study of narghile smoking in relation to cancer of the lung. Br J Cancer 16:1–6PubMedCrossRefGoogle Scholar
  24. Rastam S, Ward KD, Eissenberg T, Maziak W (2004) Estimating the beginning of the waterpipe epidemic in Syria. BMC Public Health 4:32PubMedCrossRefGoogle Scholar
  25. Roskin J, Aveyard P (2009) Canadian and English students’ beliefs about waterpipe smoking: a qualitative study. BMC Public Health 9:10PubMedCrossRefGoogle Scholar
  26. Schubert J, Hahn J, Dettbarn G, Seidel A, Luch A, Schulz TG (2011a) Mainstream smoke of the waterpipe: does this environmental matrix reveal as significant source of toxic compounds? Toxicol Lett 205:279–284PubMedCrossRefGoogle Scholar
  27. Schubert J, Kappenstein O, Luch A, Schulz TG (2011b) Analysis of primary aromatic amines in the mainstream waterpipe smoke using liquid chromatography–electrospray ionization tandem mass spectrometry. J Chromatogr A 1218:5628–5637PubMedCrossRefGoogle Scholar
  28. Smith JK, Novotny TE, Edland SD, Hofstetter CR, Lindsay SP, Al-Delaimy WK (2011) Determinants of hookah use among high school students. Nicotine Tob Res 13:565–572PubMedCrossRefGoogle Scholar
  29. Smith-Simone S, Maziak W, Ward KD, Eissenberg T (2008) Waterpipe tobacco smoking: knowledge, attitudes, beliefs, and behavior in two U.S. samples. Nicotine Tob Res 10:393–398PubMedCrossRefGoogle Scholar
  30. Stein YS, Antal MJ (1983) A study of the gas-phase pyrolysis of glycerol. J Anal Appl Pyrolysis 4:283–296CrossRefGoogle Scholar
  31. Talhout R, Opperhuizen A, van Amsterdam JG (2007) Role of acetaldehyde in tobacco smoke addiction. Eur Neuropsychopharmacol 17:627–636PubMedCrossRefGoogle Scholar
  32. Talhout R, Schulz T, Florek E, van Benthem J, Wester P, Opperhuizen A (2011) Hazardous compounds in tobacco smoke. Int J Environ Res Public Health 8:613–628PubMedCrossRefGoogle Scholar
  33. Uchiyama S, Inaba Y, Kunugita N (2010) Derivatization of carbonyl compounds with 2,4-dinitrophenylhydrazine and their subsequent determination by high-performance liquid chromatography. J Chromatogr B 879:1282–1289CrossRefGoogle Scholar
  34. Warren CW, Lea V, Lee J, Jones NR, Asma S, McKenna M (2009) Change in tobacco use among 13–15 year old between 1999 and 2008: findings from the global youth tobacco survey. Global Health Promot 16(2_suppl):38–90CrossRefGoogle Scholar
  35. Yip SH, Taylor LT, Ashraf-Khorassani M, Yu J, Borgerding MF, Coleman WM III, Bodnar JA (2010) HPLC–MS determination of acrolein and acetone generated from 13C3-labeled glycerol added to cigarette tobacco using two machine-smoking regimes. Contrib Tob Res 24:48–57Google Scholar
  36. Zurek G, Karst U (1999) Liquid chromatography–mass spectrometry method for the determination of aldehydes derivatized by the Hantzsch reaction. J Chromatogr A 864:191–197PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Jens Schubert
    • 1
  • Volkmar Heinke
    • 1
  • Jana Bewersdorff
    • 1
  • Andreas Luch
    • 1
  • Thomas G. Schulz
    • 1
    • 2
  1. 1.Department of Product SafetyGerman Federal Institute for Risk Assessment (BfR)BerlinGermany
  2. 2.Department of Chemicals SafetyGerman Federal Institute for Risk Assessment (BfR)BerlinGermany

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