Archives of Toxicology

, 83:1109 | Cite as

Quantitative determination of TEGDMA, BHT, and DMABEE in eluates from polymerized resin-based dental restorative materials by use of GC/MS

  • Mario SeissEmail author
  • Christopher Langer
  • Reinhard Hickel
  • Franz-Xaver Reichl
Genotoxicity and Carcinogenicity


This study investigated the leaching of ingredients from several commercial dental composite resins cured with LED, and immersed in methanol or water for 24 h, respectively. The composites used were: Admira Dentin (VOCO), Artemis Schmelz (Enamel) (Ivoclar Vivadent), Els extra low shrinkage (Saremco Dental), Filtek Supreme XT Dentin (3 M ESPE), Gradia Direct (GC), Venus & Venus flow (Heraeus Kulzer), and XRV Herculite Prodigy Enamel (Kerr). From each dental composite four specimens with defined structure and 100-mg net weight were made. After the polymerization process, according to manufacturer's instructions, the specimens were immersed in either 1 ml water or 1 ml methanol and incubated at 37°C for 24 h. Eluted ingredients triethyleneglycoldimethacrylate (TEGDMA), 2,6-di-tert-butyl-4-methylphenol (BHT), and 4-N,N-dimethylaminobenzoicacidethylester (DMABEE) were detected and quantified using gas chromatography–mass spectrometry (GC–MS). The amounts of the detected analytes from 100 mg polymerized composites ranged between the following values: TEGDMA: 0–0.5 mg (water), 0–1.6 mg (methanol); BHT: 0–0.03 μg (water), 0–0.11 mg (methanol); and DMABEE: 0–0.11 mg (water), 0–1.4 mg (methanol). We conclude from the results that the elution rates into methanol and water differ significantly. Furthermore, it is concluded that all the determined amounts eluting from the composites are far below toxic-relevant concentrations.


Triethyleneglycoledimethacrylate (TEGDMA) 2,6-Di-t-butyl-4-methylphenol (BHT) 4-N,N-dimethylaminobenzoic acid ethylester (DMABEE) Elution Quantification Analysis 


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Mario Seiss
    • 1
    • 2
    Email author
  • Christopher Langer
    • 1
  • Reinhard Hickel
    • 2
  • Franz-Xaver Reichl
    • 1
    • 2
  1. 1.Walther-Straub-Institute of Pharmacology and ToxicologyLudwig-Maximilians-University of MunichMunichGermany
  2. 2.Department of Operative Dentistry and PeriodontologyLudwig-Maximilians-University of MunichMunichGermany

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