Apoptosis induced by acrylamide in SH-SY5Y cells
- 431 Downloads
Acrylamide (1–5 mM) dose-dependently decreased cell viability in human neuroblastoma cells (SH-SY5Y). The caspase-3 activity and cell population in sub-G1 phase were elevated and peaked on exposure to 3 mM acrylamide, while both were less so at higher dose (4 and 5 mM). Z-VAD-fmk, a pan-caspase inhibitor, lowered the apparent cytotoxicity of acrylamide. U0126, a specific inhibitor of extracellular signal-regulated protein kinase (ERK) kinase, suppressed the elevation of caspase-3 activities as well as that of sub-G1 population. Thus, although mechanisms other than caspase-dependent apoptosis may be involved, apoptotic process seems to take place in the genesis of toxicity of acrylamide in SH-SY5Y cells through ERK pathway and activation of caspase-3.
KeywordsAcrylamide SH-SY5Y cells Caspase-3 Apoptosis ERK
We thank Dr. Tsutomu Sugiura (Department of Immunology) for his help. This work was supported in part by Grant-in Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
- Gold BG, Schaumburg HH (2000) Acrylamide. In: Spencer PS, Schaumburg HH (eds) Experimental and clinical neurotoxicology, 2nd edn. Oxford University Press, New York pp 124–132Google Scholar
- Stanciu M, Wang Y, Kentor R, Burke N, Watkins S, Kress G, Reynolds I, Klann E, Angiolieri MR, Johnson JW, DeFranco DB (2000) Persistent activation of ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures. J Biol Chem 275:12200–12206PubMedCrossRefGoogle Scholar