Archives of Toxicology

, Volume 80, Issue 3, pp 163–168

Partial purification and characterization of a novel neurotoxin and three cytolysins from box jellyfish (Carybdea marsupialis) nematocyst venom

  • J. Sánchez-Rodríguez
  • E. Torrens
  • L. Segura-Puertas
Organ Toxicity and Mechanisms


This paper describes one neurotoxin and three cytolysins isolated from the venom of the Caribbean box jellyfish Carybdea marsupialis. To assess the cytolytic and neurotoxic activity of the nematocyst venom, several bioassays were carried out, and to evaluate the effect of the toxin, the dose causing 50% lethality (LD50) was determined in vivo using sea crabs (Ocypode quadrata). The proteins with neurotoxic and cytolytic effects were isolated using low-pressure liquid chromatography. The fraction containing the neurotoxic activity was analyzed by SDS-PAGE and showed a single protein band with an apparent molecular weight of 120 kDa (CmNt). To demonstrate the neurotoxic activity of this protein, a small fraction of the purified protein was injected into a crab, and the typical convulsions, paralysis, and death provoked by neurotoxins were observed. Three fractions containing cytolysins had protein bands in SDS PAGE with apparent molecular weights of 220, 139, and 36 kDa, and their cytolytic activity was confirmed with the haemolysis assay.


Cnidaria Carybdea marsupialis Nematocysts Neurotoxins Cytolysins 


  1. Béress L, Bruhn T, Sánchez-Rodríguez J, Wachter E, Schweitz H (2000) Sea anemone Toxins, acting on Na+ channels and K+ channels: isolation and characterization. In: Rochat H, Martín-Eauclaire MF (eds) Methods and Tools in Biosciences and Medicine Animal Toxins. Birkhäuser Verlag, Switzerland, pp 31–56Google Scholar
  2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  3. Hucho F (1995) Toxins as tools in Neurochemistry. Angew Chem Int Ed Engl 34:39–50CrossRefGoogle Scholar
  4. Kass-Simon G, Scappaticci AA (2002) The behavioral and developmental physiology of nematocysts. Can J Zool 80:1772–1794CrossRefGoogle Scholar
  5. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedCrossRefGoogle Scholar
  6. Lehman AL (1980) Dosis Letal o Efectiva Media: Calculo manual fácil y rápido. Arch Far Toxicol 6:161–178Google Scholar
  7. Milla L, Segura-Puertas L, Celis L, Heimer de la Cotera E (2000) Dermatitis por contacto con Carybdea marsupialis (cnidaria cubozoa). Dermatología Rev Mex 44:167–170Google Scholar
  8. Radwan FY, Burnett JW, Bloom DA, Coliano T, Eldefrawi ME, Erderly H, Aurelian L, Torres M, Heimer-de la Cotera EP (2001) A comparison of the toxinological characteristics of two Cassiopea and Aurelia species. Toxicon 39:245–257PubMedCrossRefGoogle Scholar
  9. Rottini G, Gusman L, Parovel MA, Patriarca AL (1995) Purification and properties of a cytolytic toxin in venom of the jellyfish Carybdea marsupialis. Toxicon 33:315– 326PubMedCrossRefGoogle Scholar
  10. Rottini G, Dobrina A, Forgiarini O, Nardon E, Amirante GA, Patriarca P (1990) Identification and partial characterization of a cytolytic toxin produced by Gardnerella vaginalis. Infection and Immunity 58:3751–3758PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • J. Sánchez-Rodríguez
    • 1
  • E. Torrens
    • 1
  • L. Segura-Puertas
    • 1
  1. 1.Instituto de Ciencias del Mar y Limnología, Unidad Académica Puerto MorelosUniversidad Nacional Autónoma de MéxicoCancúnMéxico

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