Archives of Toxicology

, Volume 76, Issue 8, pp 437–441 | Cite as

Lipoic acid in combination with a chelator ameliorates lead-induced peroxidative damages in rat kidney

  • Rajagopalan Sivaprasad
  • Manickam Nagaraj
  • Palaninathan Varalakshmi
Inorganic Compounds

Abstract.

The deleterious effect of lead has been attributed to lead-induced oxidative stress with the consequence of lipid peroxidation. The present study was designed to investigate the combined effect of DL-α-lipoic acid (LA) and meso-2,3-dimercaptosuccinic acid (DMSA) on lead-induced peroxidative damages in rat kidney. The increase in peroxidated lipids in lead-poisoned rats was accompanied by alterations in antioxidant defence systems. Lead acetate (Pb, 0.2%) was administered in drinking water for 5 weeks to induce lead toxicity. LA (25 mg/kg body weight per day i.p) and DMSA (20 mg/kg body weight per day i.p) were administered individually and also in combination during the sixth week. Nephrotoxic damage was evident from decreases in the activities of γ-glutamyl transferase and N-acetyl β-D-glucosaminidase, which were reversed upon combined treatment with LA and DMSA. Rats subjected to lead intoxication showed a decline in the thiol capacity of the cell, accompanied by high malondialdehyde levels along with lowered activities of catalase, superoxide dismutase, glutathione peroxidase and glutathione metabolizing enzymes (glutathione reductase, glucose-6-phosphate dehydrogenase, glutathione-S-transferase). Supplementation with LA as a sole agent showed considerable changes over oxidative stress parameters. The study has highlighted the combined effect of both drugs as being more effective in reversing oxidative damage by bringing about an improvement in the reductive status of the cell.

Lead acetate DL-α-Lipoic acid, Meso-2,3-dimercaptosuccinic acid Lipid peroxidation Antioxidants 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Rajagopalan Sivaprasad
    • 1
  • Manickam Nagaraj
    • 1
  • Palaninathan Varalakshmi
    • 1
  1. 1.Department of Medical Biochemistry, Dr. A.L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras (Taramani), Chennai 600 113, India

Personalised recommendations