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Archives of Toxicology

, Volume 75, Issue 11–12, pp 695–702 | Cite as

Novel action of lignans isolated from Hernandia nymphaeifolia on Ca2+ signaling in human neutrophils

  • Yu-Ying Chao
  • Warren Su
  • Chung-Ren Jan
  • Ying-Chin Ko
  • Jih-Jung Chen
  • Jin-Shiung Cheng
  • Chun-Peng Liu
  • Yuk-Keung Lo
  • Kang-Ju Chou
  • Kam-Chung Lee
  • Wei-Chung Chen
  • Ih-Sheng Chen
Molecular Toxicology

Abstract.

The effects of five lignans (epi-aschantin, epi-magnolin, epi-yangambin, deoxypodophyllotoxin, yatein) isolated from Hernandia nymphaeifolia (Presl.) Kubitzki (Hernandiaceae) on intracellular Ca2+ levels ([Ca2+]i) in human neutrophils were investigated by using fura-2 as a fluorescent probe. In both Ca2+-containing and Ca2+-free media, the lignans (50–100 µM) did not alter basal [Ca2+]i but inhibited the [Ca2+]i increase induced by platelet activating factor (PAF, 10 µM), leukotriene B4 (LTB4, 0.2 µM), and thapsigargin (1 µM) to different extents. In Ca2+-free medium, after depleting stores of Ca2+ with PAF, LTB4 or thapsigargin, addition of 3 mM Ca2+ induced Ca2+ influx. Each of the lignans (50–100 µM) caused 39–89% inhibition of PAF-induced Ca2+ influx; whereas only epi-aschantin was able to inhibit LTB4- and thapsigargin-induced Ca2+ influx by 54–79%. Together, the results suggest that in human neutrophils, these lignans did not alter basal [Ca2+]i but inhibited Ca2+ movement induced by Ca2+ mobilizing agents.

Calcium mobilization Calcium signaling Lignans Neutrophils Human 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Yu-Ying Chao
    • 1
  • Warren Su
    • 2
  • Chung-Ren Jan
    • 3
  • Ying-Chin Ko
    • 4
  • Jih-Jung Chen
    • 5
  • Jin-Shiung Cheng
    • 6
  • Chun-Peng Liu
    • 6
  • Yuk-Keung Lo
    • 6
  • Kang-Ju Chou
    • 6
  • Kam-Chung Lee
    • 6
  • Wei-Chung Chen
    • 7
  • Ih-Sheng Chen
    • 1
  1. 1.Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan 807Taiwan
  2. 2.Department of Pediatrics, Pao-Chien General Hospital, PingtungTaiwan
  3. 3.Department of Medical Education and Research, Kaohsiung Veterans General Hospital, KaohsiungTaiwan
  4. 4.Institute of Medicine, School of Medicine, Kaohsiung Medical University KaohsiungTaiwan
  5. 5.Department of Pharmacy, Tajen Institute of Technology, PingtungTaiwan
  6. 6.Department of Medicine, Kaohsiung Veterans General Hospital, KaohsiungTaiwan
  7. 7.Department of Surgery, Ping Tung Christian Hospital, PingtungTaiwan

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